
%0 Journal Article
%A Anthony, J. 
%A McLean, C. B. 
%A Lawrie, A. C. 
%T <emph type="2">In vitro</emph> propagation of <emph type="2">Epacris impressa</emph> (Epacridaceae) and the effects of clonal material
%J Australian Journal of Botany
%D 2000/03/11
%V 48
%N 2
%P 215-221
%K 
%R https://doi.org/10.1071/BT98026
%U https://www.publish.csiro.au/paper/BT98026 
%X A system of micropropagation has been developed for Epacris impressa Labill. (pink heath) (Epacridaceae), the floral emblem of Victoria, Australia. Only explants from glasshouse-grown plants treated with 1.2 g L&#8211;1 mancozeb were established successfully in vitro. Shoot material was very sensitive to surface-sterilisation, with 0.5% NaOCl for 5 min being optimal. Multiple shooting was induced optimally on Woody Plant Medium (WPM, Lloyd and McCown 1980) with 12&#8211;25 &#181;M of the cytokinin 2iP (6-(&#947;,&#947;-dimethylallylamino) purine). Inclusion of the auxin IBA (indole-3-butyric acid) induced callus and reduced shooting. Rooting in vitro was greatest (up to 40%) with half-strength WPM and 16 &#181;M IBA. Clones from individual plants varied in multiple shooting response to 2iP (0&#8211;49 &#181;M) and root induction response to auxins (IBA and NAA (&#945;-naphthaleneacetic acid), 0&#8211;43 &#181;M). These results suggest that explant materials are the main determinant of success in in vitro propagation and that they require individual optimisation of treatments to maximise shoot and root formation. 
