
%0 Journal Article
%A Gulline, HF 
%A Walker, R 
%T The regeneration of severed pea apices
%J Australian Journal of Botany
%D 1957/11/01
%V 5
%N 2
%P 129-136
%K 
%R https://doi.org/10.1071/BT9570129
%U https://www.publish.csiro.au/paper/BT9570129 
%X Successful graft unions between meristematic tissues have been made in apices of the pea, Pisum sativurn L. A technique for preventing desiccation of apices during and after the operations was developed. Apical segments ranging from about 200&micro; down to 50&micro;. in depth were successfully grafted back to their parent plant. The subsequent development of grafted apices was completely normal. These experiments show that a complete shoot can be regenerated from an apical segment less than one-thousandth of a cubic millimetre in volume, containing about 600 cells. This is smaller than any graft previously recorded. 
