
TY  - JOUR
A1  - PGoh, P 
A1  - White, IG 
T1  - Control of Ram Sperm Adenylate Cyclase by Divalent Cations
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1988
VL  - 41
IS  - 3
SP  - 377
EP  - 386
KW  - 
UR  - https://doi.org/10.1071/BI9880377 
N2  - The adenylate cyclase activity of ram sperm increased on freeze-thawing and the enzyme was stable at O°C. Its activity was stimulated by Mn2+, Zn2+, Co2+, Mg2+ and Ca2+ in descending order of activity. The enzyme was insensitive to fluoride when Mn2+ concentration was in excess. Mn2+_ stimulated enzyme activity was decreased by the simultaneous addition of Co2+, or Cd2+, or Ni2+, and particularly Cu2+. Sulfhydryl compounds (viz. dithiothreitol, glutathione, dithiocarbamate, 2- mercaptoethanol, ergothioneine and cysteine) and chelating agents (viz. D-penicillamine and 8-hydroxyquinoline) were effective, to varying degrees, in overcoming the inhibition by Cu2+. Ca2+ augmented the stimulatory effect of Mg2+, Co2+, Zn2+ and Mn2+ on enzyme activity. 
ER  -

