
%0 Journal Article
%A PGoh, P 
%A White, IG 
%T Control of Ram Sperm Adenylate Cyclase by Divalent Cations
%J Australian Journal of Biological Sciences
%D 1988/09/01
%V 41
%N 3
%P 377-386
%K 
%R https://doi.org/10.1071/BI9880377
%U https://www.publish.csiro.au/paper/BI9880377 
%X The adenylate cyclase activity of ram sperm increased on freeze-thawing and the enzyme was stable at O°C. Its activity was stimulated by Mn2+, Zn2+, Co2+, Mg2+ and Ca2+ in descending order of activity. The enzyme was insensitive to fluoride when Mn2+ concentration was in excess. Mn2+_ stimulated enzyme activity was decreased by the simultaneous addition of Co2+, or Cd2+, or Ni2+, and particularly Cu2+. Sulfhydryl compounds (viz. dithiothreitol, glutathione, dithiocarbamate, 2- mercaptoethanol, ergothioneine and cysteine) and chelating agents (viz. D-penicillamine and 8-hydroxyquinoline) were effective, to varying degrees, in overcoming the inhibition by Cu2+. Ca2+ augmented the stimulatory effect of Mg2+, Co2+, Zn2+ and Mn2+ on enzyme activity. 
