
TY  - JOUR
A1  - Annison, Geoffrey 
A1  - Couperwhite, lain 
T1  - Composition of Alginate Synthesized during the Growth Cycle of Pseudomonas aeruginosa
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1987
VL  - 40
IS  - 4
SP  - 435
EP  - 442
KW  - 
UR  - https://doi.org/10.1071/BI9870435 
N2  - A mucoid P. aeruginosa isolated from the sputum of a cystic fibrosis patient was grown in batch culture on a complex medium. During the growth cycle the amount of alginate produced was estimated and its composition was determined by proton magnetic resonance (IH-n.m.r) spectroscopy. Exopolysaccharide production occurred mainly during the exponential phase of growth. The alginate samples isolated varied little in composition and were characterized by being highly acetylated, high mannuronate (0.83 - 0.93 mole fraction) polymers. Guluronate was present only within heteropolymeric regions of the polysaccharides which all displayed a complete absence of polyguluronate. Ca2 + ion supplementation of the medium was not observed to increase the levels of guluronate in the algi nates produced. 
ER  -

