
%0 Journal Article
%A Grossman, G 
%A O'Sullivan, WJ 
%T Eastern Grey Kangaroo Muscle Creatine Kinase
%J Australian Journal of Biological Sciences
%D 1981/07/01
%V 34
%N 3
%P 269-282
%K 
%R https://doi.org/10.1071/BI9810269
%U https://www.publish.csiro.au/paper/BI9810269 
%X Creatine kinase has been purified to homogeneity from skeletal muscle of the eastern grey kangaroo, Macropus giganteus. The procedure included ethanol fractionation followed by chromatography on DEAE-cellulose. The enzyme had a molecular weight of approximately 86 000 with two subunits of 43 500. Two sulfhydryl groups were determined for the intact molecule and a further four on unfolding. Under standardized conditions, the metal ion specificity was determined as MgADP- > MnADP- > CoADP-, CaADP-; and the substrate specifiCity as MgADP- > MgdADP- > MgGDP- > MgXDP-. 
