
TY  - JOUR
A1  - GR Hurrell, John 
A1  - M Malouf, PauI 
A1  - Conway, Richard 
T1  - A New Insulin Derivative for the Preparation of Insulin-Enzyme Conjugates: An Application in the Enzyme Immunoassay of Insulin
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1980
VL  - 33
IS  - 6
SP  - 633
EP  - 642
KW  - 
UR  - https://doi.org/10.1071/BI9800633 
N2  - A new insulin derivative, mercaptobutyrimidyl pyridine disulfide insulin, was prepared under conditions which directed the reaction preferentially through the terminal (X-amino group of the A-chain. The purified monosubstituted insulin exhibited only a marginal decrease in antigenicity as measured by radioimmunoassay; there was, however, a significant reduction in biological activity in the mouse convulsion assay. Conjugation of the insulin derivative to human placental alkaline phosphatase was carried out via a thiol interchange reaction and the resulting conjugate was active in a double-antibody, solid-phase enzyme immunoassay for insulin. 
ER  -

