
%0 Journal Article
%A L Skotnicki, Mary 
%A G Rolfe, Barry 
%T Bacterial and Bacteroid Properties of Mutants of Rhizobium Trifolii Strain T1 Uncoupled in Oxidative Phosphorylation
%J Australian Journal of Biological Sciences
%D 1979/10/01
%V 32
%N 5
%P 501-518
%K 
%R https://doi.org/10.1071/BI9790501
%U https://www.publish.csiro.au/paper/BI9790501 
%X Using the neomycin-resistance selection technique, mutants uncoupled in oxidative phosphorylation were isolated in R. tri/olii strain Tl. These mutants had no detectable ATP-synthesizing activity and between 8 and 20% of the ATP-degrading activity of their parent strain in the bacterial form. The mutants formed nitrogen-fixing nodules on red, white, and subterranean clovers, and bacteroids isolated from white clover nodules had a high level of ATPase activity of about 70% of that found in bacteroids of their parental strain. The possibility that a new ATPase complex is synthesized when the bacteria differentiate to form bacteroids within the nodule is discussed. 
