
TY  - JOUR
A1  - Bilton, RJ 
A1  - Moore, NW 
T1  - Factors Affecting the Viability of Frozen Stored Cattle Embryos
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1979
VL  - 32
IS  - 1
SP  - 101
EP  - 108
KW  - 
UR  - https://doi.org/10.1071/BI9790101 
N2  - Cattle morulae and blastocysts were frozen, stored in liquid N2 and then warmed and cultured in vitro at 37· 5°C. They were frozen and stored in Dulbecco phosphate buffer enriched with 25 % cattle serum (DB+S)and containing 1·5 M dimethylsulphoxide (DMSO) or 1·0 M glycerol. Embryos were cooled at rates of 0 ·13, 0 ·15 or O· 30°Cjmin and warmed at rates of 1 ·2, 2·2 or 4· 6°C/min (measured over the range 0 to - 50°C). Warming and cooling between 0 and 37· 5°C was at a rate of 0·7°C/min and, after warming, embryos were cultured in DB+S for 12-18 h. A number of embryos were transferred after culture to recipient cows. 
ER  -

