
TY  - JOUR
A1  - Sinclair, MI 
A1  - Morgan, AF 
T1  - Transformation of Pseudomonas Aeruginosa Strain Pao With Bacteriophage and Plasmid DNA
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1978
VL  - 31
IS  - 6
SP  - 679
EP  - 688
KW  - 
UR  - https://doi.org/10.1071/BI9780679 
N2  - A procedure has been developed which allows transformation of P. aeruginosa strain PAD with plasmid and bacteriophage DNA at 'a frequency of 10- 6 per recipient cell. The method is similar in outline to. that developed for Escherichia coli. It involves growing the recipient cells to 3-5 x 108 per ml in nutrient broth, washing the cells with 0·1 M MgCI2 , resuspending in 0 ·175 M CaC12 for 20 min, exposing to DNA for 1 h and then heat pulsing at 42°C for 1 min. Some plasmid markers are expressed immediately, whereas others require time for phenotypic expression. 
ER  -

