
TY  - JOUR
A1  - Culvenor, CCJ 
A1  - Beck, AB 
A1  - Clarke, M 
A1  - Cockrum, PA 
A1  - Edgar, JA 
A1  - Frahn, JL 
A1  - Jago, MV 
A1  - Lanigan, GW 
A1  - Payne, AL 
A1  - Peterson, JE 
A1  - Petterson, DS 
A1  - Smith, LW 
A1  - White, RR 
T1  - Isolation of Toxic Metabolites of Phomopsis leptostromiformis Responsible for Lupinosis
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1977
VL  - 30
IS  - 4
SP  - 269
EP  - 278
KW  - 
UR  - https://doi.org/10.1071/BI9770269 
N2  - Two metabolites of P. leptostromijormis (phomopsins A and B) have been isolated as a crystalline mixture from a culture of the fungus on lupin seed. The mixture has been shown to be capable of inducing lupinosis in sheep and in young rats. Key steps in the isolation were the transfer of the phomopsins from crude aqueous solution to tetrahydrofuran and chromatography on macroreticular polystyrene resin. The bioassays used in monitoring fractions were based on inhibition of cell cultures and the mitosis-arresting effect of the metabolites on liver cells in vivo. 
ER  -

