
TY  - JOUR
A1  - Jones, RC 
A1  - Foote, RH 
T1  - Effect of Osmolality and Phosphate, 'Tris', 'Tes', 'Mes', and 'Hepes' Hydrogen Ion Buffers on the Motility of Bull Spermatozoa Stored At 37 Or 5°C
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1972
VL  - 25
IS  - 5
SP  - 1047
EP  - 1056
KW  - 
UR  - https://doi.org/10.1071/BI9721047 
N2  - Bull spermatozoa survived incubation, in unstoppered tubes at 5 and 37°C, better in solutions of 250 m-05molesJkg than in solutions of higher or lower osmolali-ties regardless of the concentrations of sodium chloride or citrate in the solutions relative to the concentrations of phosphate, Tris, TES, MES, or HEPES. When buffer solutions contributed 120 m-osmolesJkg to a total diluent strength of 250 m-osmolesJkg, the ranking from best to worst on the basis of survival of spermatozoa was: MES, HEPES, TES, Tris, and phosphate. Comparisons when 50 mM con-centrations of the buffers were used showed the same ranking except that HEPES was as good as.MES. 
ER  -

