
%0 Journal Article
%A Jones, RC 
%A Foote, RH 
%T Effect of Osmolality and Phosphate, 'Tris', 'Tes', 'Mes', and 'Hepes' Hydrogen Ion Buffers on the Motility of Bull Spermatozoa Stored At 37 Or 5°C
%J Australian Journal of Biological Sciences
%D 1972/10/01
%V 25
%N 5
%P 1047-1056
%K 
%R https://doi.org/10.1071/BI9721047
%U https://www.publish.csiro.au/paper/BI9721047 
%X Bull spermatozoa survived incubation, in unstoppered tubes at 5 and 37°C, better in solutions of 250 m-05molesJkg than in solutions of higher or lower osmolali-ties regardless of the concentrations of sodium chloride or citrate in the solutions relative to the concentrations of phosphate, Tris, TES, MES, or HEPES. When buffer solutions contributed 120 m-osmolesJkg to a total diluent strength of 250 m-osmolesJkg, the ranking from best to worst on the basis of survival of spermatozoa was: MES, HEPES, TES, Tris, and phosphate. Comparisons when 50 mM con-centrations of the buffers were used showed the same ranking except that HEPES was as good as.MES. 
