
%0 Journal Article
%A Hopgood, MF 
%A Walker, DJ 
%T Succinic Acid Production by Rumen Bacteria. III. Enzymic Studies on the Formation of Succinate by Ruminococcus Flavefaciens
%J Australian Journal of Biological Sciences
%D 1969/12/01
%V 22
%N 6
%P 1413-1424
%K 
%R https://doi.org/10.1071/BI9691413
%U https://www.publish.csiro.au/paper/BI9691413 
%X Enzymes involved in succinic acid production by strain C of R. ftavefacien8 were investigated in cell-free extracts. The results indicate that phosphoenolpyruvate is carboxylated by a phosphoenolpyruvate carboxykinase which uses GDP as phosphate acceptor, and that the oxaloacetate so formed is converted to succinate via a DPNH-dependent malate dehydrogenase, a fumarate hydratase, and a DPNH-dependent fumarate reductase. Succinate dehydrogenase activity was also observed which differed markedly from fumarate reductase in that DPN+ was not reduced and in inhibition characteristics. 
