
%0 Journal Article
%A O'shea, T 
%A Wales, RG 
%T Further Studies of The Deep Freezing of Rabbit Spermatozoa In Reconstituted Skim Milk Powder
%J Australian Journal of Biological Sciences
%D 1969/06/01
%V 22
%N 3
%P 709-720
%K 
%R https://doi.org/10.1071/BI9690709
%U https://www.publish.csiro.au/paper/BI9690709 
%X Using motility as the criterion of survival of spermatozoa, a method for the deep-frozen storage of rabbit semen was developed. The factors found to give optimum revival were the presence of 14% (v/v) dimethylsulphoxide during freezing, the inclusion of fructose in the diluent, and cooling to 5°C in an isotonic diluent while freezing in a hypertonic diluent. 
