
%0 Journal Article
%A Spencer, D 
%A Unt, H 
%T Biochemical and Structural Correlations in Isolated Spinach Chloroplasts Under Isotonic and Hypotonic Conditions
%J Australian Journal of Biological Sciences
%D 1965/04/01
%V 18
%N 2
%P 197-210
%K 
%R https://doi.org/10.1071/BI9650197
%U https://www.publish.csiro.au/paper/BI9650197 
%X Methods are described for obtaining preparations of isolated spinach chloro-plasts which are predominantly either "whole", i.e. retain the outer limiting membrane, or "naked", i.e. with the outer membrane removed. The whole chloro-plasts are shown to retain more of their soluble components as evidenced by higher endogenous capacities for CO.-fixation, photophosphorylation, and light-dependent reduction of triphosphopyridine nucleotide. The isolation is also described of whole chloroplasts whose peripheral jackets have retained the property of mobility seen in living cells. 
