
%0 Journal Article
%A Lee, JW 
%A Tracey, MV 
%A Winzor, DJ 
%A Wrigley, CW 
%A Zentner, H 
%T Properties of Gluten Fractions Prepared by Ion-Exchange Chromatography on Carboxymethyl-Cellulose
%J Australian Journal of Biological Sciences
%D 1963/09/01
%V 16
%N 3
%P 717-726
%K 
%R https://doi.org/10.1071/BI9630717
%U https://www.publish.csiro.au/paper/BI9630717 
%X Seven fractions obtained from wheat gluten by chromatography on carboxy~ methyl-cellulose were studied by ultracentrifugation, gel electrophoresis, chemical, and N-terminal amino acid analysis. On rechromatography, five fractions eluted by sodium chloride behaved as distinct entities. illtracentrifuge experiments indicated that four of these were each undergoing rapid, reversible association. Several N-terminal amino acids were found in each of the fractions which, moreover, could be resolved by the gel technique into a number of electrophoretic bands, some bands being common to those of neighbouring fractions. Total nitrogen values showed the chromatographic samples to be essentially free from non-protein material. 
