
%0 Journal Article
%A Simmond, DH 
%A Winzor, DJ 
%T Chromatographic Fractionation of the Acetic Acid Soluble Proteins of Wheat Flour on Carboxymethyl-Cellulose
%J Australian Journal of Biological Sciences
%D 1961/12/01
%V 14
%N 4
%P 690-699
%K 
%R https://doi.org/10.1071/BI9610690
%U https://www.publish.csiro.au/paper/BI9610690 
%X The acetic acid soluble proteins extracted from wheat flour have been separated into 11 fractions by a chromatographic procedure using columns of carboxymethyl- cellulose, equilibrated with O· 005M acetate buffer, pH 4·1. Under theso conditions one fraction passes unretarded through the column. Eight further fractions are eluted at pH 4· 1 by employing a gradient to O· 2M N aCl in the presence of 1M dimethyl formamide (DMF) which prevents protein precipitation at ionic strengths greater than 0·03. 
