
TY  - JOUR
A1  - Joklik, W 
T1  - Studies on the Nitrate Reductase of Escherichia Coli in the Cell-Free State
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1950
VL  - 3
IS  - 1
SP  - 28
EP  - 44
KW  - 
UR  - https://doi.org/10.1071/BI9500028 
N2  - A method of purifying the nitrate reductase obtained by crushingEscherichia coli cells in a ground glass mill is described, involving elution atpH 9.5 and precipitation into slightly acidified acetone, followed by treatmentwith phosphate buffer ( pH 8.0) and dialysis. Methods of estimating theactivity of the enzyme without recourse to coupling with other enzymes areoutlined, viz.: (a) the oxidation time method; (b) the use of photochemicallyreduced methylene blue as hydrogen donor. 
ER  -

