
%0 Journal Article
%A Joklik, W 
%T Studies on the Nitrate Reductase of Escherichia Coli in the Cell-Free State
%J Australian Journal of Biological Sciences
%D 1950/03/01
%V 3
%N 1
%P 28-44
%K 
%R https://doi.org/10.1071/BI9500028
%U https://www.publish.csiro.au/paper/BI9500028 
%X A method of purifying the nitrate reductase obtained by crushingEscherichia coli cells in a ground glass mill is described, involving elution atpH 9.5 and precipitation into slightly acidified acetone, followed by treatmentwith phosphate buffer ( pH 8.0) and dialysis. Methods of estimating theactivity of the enzyme without recourse to coupling with other enzymes areoutlined, viz.: (a) the oxidation time method; (b) the use of photochemicallyreduced methylene blue as hydrogen donor. 
