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Australian Journal of Biological Sciences Australian Journal of Biological Sciences Society
Biological Sciences
RESEARCH ARTICLE

Control of Ram Sperm Adenylate Cyclase by Divalent Cations

P PGoh and IG White

Australian Journal of Biological Sciences 41(3) 377 - 386
Published: 1988

Abstract

The adenylate cyclase activity of ram sperm increased on freeze-thawing and the enzyme was stable at O°C. Its activity was stimulated by Mn2+, Zn2+, Co2+, Mg2+ and Ca2+ in descending order of activity. The enzyme was insensitive to fluoride when Mn2+ concentration was in excess. Mn2+_ stimulated enzyme activity was decreased by the simultaneous addition of Co2+, or Cd2+, or Ni2+, and particularly Cu2+. Sulfhydryl compounds (viz. dithiothreitol, glutathione, dithiocarbamate, 2- mercaptoethanol, ergothioneine and cysteine) and chelating agents (viz. D-penicillamine and 8-hydroxyquinoline) were effective, to varying degrees, in overcoming the inhibition by Cu2+. Ca2+ augmented the stimulatory effect of Mg2+, Co2+, Zn2+ and Mn2+ on enzyme activity.

https://doi.org/10.1071/BI9880377

© CSIRO 1988

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