Metabolic Properties of Mouse Uterine Endometrial Cells after Isolation with Collagenase

Abstract

Uterine horns from unmated, pseudopregnant (days 1-5) and pregnant (days 4 and 5) mice were dissected and treated with collagenase (5 mg/ml) in a Ringer solution to isolate viable endometrial cells with maximal recovery of the luminal epithelium.