A Highly Repeated DNA From the Genome of the Wallaroo (Macro pus Robustus Robustus)

Abstract

The major satellite of M. r. robustus DNA has been isolated in a Ag+ -CS2S04 gradient.has a of 1· 710 gjcm3 compared with 1· 697 gjcm3 for the bulk of the DNA, and accounts for about 10% of the total DNA. Sequence heterogeneity within the satellite was shown by an increase in density to 1·715 gjcm3 and by a reduction of 12°C in the temperature of denaturation (Tm) after renaturation. The satellite was found to occur in the centromeric regions of all chromosomes. This pattern of distribution was essentially duplicated in the genomes of other members of the wallaroo group. A polymorphism for a major block of the sequences on chromosome 5 occurred in both M. r. erubescens and M. antilopinus.