Amino Acid Sequences Containing Cysteine or Half-Cystine Residues in ß-Glucuronidase

Abstract

Amino acid analysis of oxidized or reduced and carboxymethylated p-glucuronidase have shown the presence of 24 cysteic acid or S-carboxymethylcysteine residues respectively per mole of the tetrameric enzyme. Titration of sulfhydryl groups gave eight cysteine residues, and by difference 16 half-cystine residues per mole.