Effect of Rec-2+ on the Formation of Double Mutant Recombinants in Neurospora Crassa

Abstract

In crosses between his-3(K874) and ad-3A(K118), the presence of rec-2+ reduces the frequency of formation of both prototrophic recombinants (his-3+ ad-3A +) and double mutant recombinants (his-3 ad-3A) to a similar degree. This is consistent with the hypothesis that the product of the rec-2+ gene acts to block the initiation of recombination events and indicates that rec-2+ does not determine the frequency of formation of prototrophic recombinants by influencing the type of recombination product which predominates.