Location of a High-sulphur Protein in Developing Wool Follicles Using Peroxidase-Labelled Antibody

Abstract

Antiserum to a high-sulphur protein (SCMK -B2) from wool was purified to obtain the immunoglobin G fraction, and this was labelled with horse-radish peroxidase. The labelled antibody was then applied to sections of sheep skin embedded in glycol methacrylate in order to locate the distribution of high-sulphur protein within the lower hair shaft. Regions containing the highest concentration of high-sulphur protein were the cuticle of the hair shaft and the keratogenous zone of the cortex.