Further Studies on the Accumulation of Energy Substrates by Two-cell Mouse Embryos

Abstract

The accumulation of substrate carbon by two-cell mouse embryos after incubation for 30 min in isotopically labelled substrates was compared by two different methods. The embryos were centri-fuged either through an isotonic sucrose solution to free them from radioactive medium before coun-ting or in radioactive medium in a capillary tube when substrate uptake was calculated from the relative counts in packed embryos and incubation medium.