Dehydration of Macromolecules II. Protective Effects of Certain Anions on Ribulosediphosphate Carboxylase Subjected to Low Water Potentials in Vitro

Abstract

Studies of the instability of ribulosediphosphate carboxylase at low water potentials, generated in a pressure-membrane apparatus, have been continued. The presence of phosphate, pyrophosphate, citrate, or ribulose diphosphate during treatment protects the protein against fragmentation. Tris-sulphate, sodium sulphate, tetra-alkyl ammonium salts, or polyvinylpyrrolidone do not afford protection.