Studies on lolium multiflorum endosperm in tissue culture I. Nutrition

Abstract

Stocks of L. multiflorum endosperm callus have been maintained in liquid suspension culture on a modified White's medium for 5 years. The mean doubling time under the conditions used is 3· 2 days. Best growth is obtained on sucrose; fructose and glucose are good carbon sources, whereas growth is only moderate on an equimolar mixture of both.