Quantitative Changes in Mouse Liver Ultrastructure Following Cortisone and Insulin Administration

Abstract

Linear analysis of whole cell montages provided the basis for determining the following in mouse liver parenchymal cells: the cytoplasmic volume fraction of mitochondria, lysosomes, lipid, glycogen, and cytoplasmic matrix; the membrane profile concentration; and mitochondrial number and dimensions (including a shape coefficient). Included are a number of studies of the validity of the methods used, some new statistical considerations, and a new, more objective method of determining mitochondrial shape.