Fungal Cellulases XVI. Alkane-1, O-Diols As Acceptors for the ?-Glucosidase of Stachybotrys Atra

Abstract

Using hexane-l,6-diol as an exemplar, methods are developed for estimating the kinetics of the reaction of enzyme and phenyl ,B-D-glucopyranoside with an added acceptor. The effects of pH on the rate of decomposition of the enzymeglucoside- hexanediol complex and on the Micl;laelis constant of the partial reaction of enzyme-hexanediol with glucoside are considerably different from those for the water complex. Some less certain deductions are also made for the reaction of enzyme with butane-l,4-diol and p-nitrophenyl ,B-D-glucopyranoside.