Microelectrode Experiments on Nitella

Abstract

Tubular glass micro electrodes containing concentrated KCl solutions have been inserted into internodal cells of Nitella, under adequate visual control, and the electric potentials of cytoplasm and vacuole measured. The potential of the cytoplasm is found to be close to that of the sap. For one of the groups of cells studied, this potential was - 160 m V referred to the solution bathing the cell when this was O· 0001N KC!. Changes in the KCI concentration of this solution caused the potential of the cytoplasm to change in a similar way to that of the sap.