Preservation of Rabbit, Sheep, and Ox Red Cells At -79°C

Abstract

Observations made on the freezing of red cells at -79°C show that equilibration of the cells with a glycerol diluent reduces the amount of haemolysis in rabbit blood but increases it in sheep and ox blood. When ethylene glycol is substituted for glycerol, no effect of equilibration is detectable in rabbit blood, but haemolysis is increased in sheep and ox blood.