The Intermediary Metabolism of Nematode Parasites

Abstract

The oxygen consumption of brei or mince prepared from Nematodirusfilicollis, N. spathiger, Ascaridia galli, and Neoaplectana glaseri was stimulatedby the addition of pyruvate, a-ketoglutarate, succinate, fumarate, malate, andoxaloacetate. Citrate did not stimulate respiration. Malonate, arsenate, arsenite,pyrophosphate, and azide inhibited respiration. When brei from Nematodirusspp. and Ascaridia galli were fortified with coenzymes, the inhibition due tomalonate was decreased by adding intermediates of the tricarboxylic acidcycle, and the accumulation of succinate in the malonate-poisoned brei was increasedwhen fumarate, fumarate plus pyruvate, or citrate was added.