
%0 Journal Article
%A Lodge, GM 
%T Seed and seedling studies of three <I>Trifolium subterraneum</I> var. <I>brachycalycinum</I> lines, in a predominantly summer rainfall environment
%J Australian Journal of Experimental Agriculture
%D 1995/12/01
%V 35
%N 8
%P 1101-1108
%K 
%R https://doi.org/10.1071/EA9951101
%U https://www.publish.csiro.au/paper/EA9951101 
%X Field experiments were conducted in a predominantly summer rainfall environment to investigate burr burial, seed production, seed characteristics, seedling emergence and survival, and the effects of time of sowing on 3 Trifolium subterraneum var. brachycalycinum lines (cv. Clare and 2 local lines). Each line produced more surface than buried burrs; surface burrs were 59% (range 56-62%) of the total number of burrs recovered and produced 59% of the total seed number. Numbers of seeds per burr were similar for surface and buried burrs, however, buried seeds were 0.97 mg heavier (P<0.05) than surface seeds. Storage for 5 months at 25/60¦C decreased hardseed content of surface seed by 50% and buried seed by 70%. Surface and buried seeds stored at 25/25¦C for 3 months prior to sowing in trays had a total emergence of <10%, compared with 70% emergence for seeds stored at 25/60¦C before sowing. These emergence differences reflected their levels of hardseededness. Numbers of seeds recovered from the soil were not significantly different among lines, declining from about 4200 seeds/m2 after initial seed set to 150 seeds/m2 by the following winter, a 97% decrease. Seed production in the second year increased seed reserves to about 8730 seeds/m2. With no further seed production, levels had declined by 93% in June 1990 and by 99% in May 1991. These data confirm the importance of annual seed production for persistence. Total seedling emergence in summer-autumn accounted for only 10% of the estimated seed production in each year. Seedling survival in summer-autumn 1988-89 was 92.7%, more than double the survival in 1989-90. The effect of sowing time on flowering was always significant, with time to first flower being highest (196 days) for the earliest sowing in March (P<0.05), progressively decreasing (P<0.05) to 108 days for the latest sowing in July. In March, April and May sowings, inflorescence numbers on the first day of flowering were similar at about 120/m2, but increased markedly (P<0.05) for sowing in June or July. However, for the March and July sowings, number of inflorescences at the 9 November 1990 count, were lowest (P<0.05). May or June sowings had the highest number of burrs and seeds (P<0.05), indicating that these may be the best sowing times for maximum seed production in these Trifolium subterraneum var. brachycalycinum lines. 
