Isolation and characterisation of microsatellites for the endangered Slater’s skink, Liopholis slateri (Squamata : Scincidae), via next-generation sequencing
Michael G. Gardner A B G , Mina H. Ansari A C , Claire E. Treilibs A D E , Angharad Johnston A , Chris R. Pavey E F and C. Michael Bull AA College of Science and Engineering, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
B Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia.
C Present address: Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
D Flora and Fauna Division, Northern Territory Department of Environment and Natural Resources, PO Box 1120, Alice Springs, NT 0871, Australia.
E CSIRO Land and Water, PO Box 2111, Alice Springs, NT 0871, Australia.
F Present address: CSIRO Land and Water, PMB 44, Winnellie, NT 0822, Australia.
G Corresponding author. Email: michael.gardner@flinders.edu.au
Australian Journal of Zoology 65(3) 200-202 https://doi.org/10.1071/ZO17053
Submitted: 23 August 2017 Accepted: 31 October 2017 Published: 20 November 2017
Abstract
We characterised 14 new polymorphic microsatellite loci for the endangered lizard Liopholis slateri. Initially, 454 shotgun sequencing was used to identify 46 loci, which were trialled for amplification. Subsequently, 14 of these loci were screened for variation in 21 individuals from scat-derived DNA samples collected from Owen Springs Reserve in central Australia. All 14 loci were polymorphic, with observed heterozygosity ranging from 0.19 to 0.86 and the number of alleles per locus ranging from 2 to 10. These loci will be useful in understanding the genetic variation and connectivity within and among extant L. slateri populations.
Additional keywords: 454 GS-FLX, scat DNA, shotgun sequencing.
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