Isolation and characterisation of 16 novel polymorphic microsatellite loci for the Eurasian coot (Fulica atra)
Tianshu Lv A C , Wanchao Zhu A C , Shuyu Xue A , Huanxin Zhang B , Xiaodong Gao A , Lidong Wang A and Honghai Zhang A DA College of Life Science, Qufu Normal University, Qufu, QF 273100, China.
B College of Marine Life Sciences, Ocean University of China, Qingdao, QD 266100, China.
C These authors contributed equally to this work.
D Corresponding author. Email: zhanghonghai67@126.com
Australian Journal of Zoology 64(6) 430-433 https://doi.org/10.1071/ZO16077
Submitted: 31 October 2016 Accepted: 24 March 2017 Published: 13 April 2017
Abstract
To study the genetic diversity of the Eurasian coot (Fulica atra), 16 novel microsatellites were isolated and characterised using a (AC)12-enriched genomic library. Polymorphism of each locus was detected in 19 individuals from Weishan Lake and Zhaoyang Lake, both located in Shandong Province, China. These microsatellite loci revealed 3–12 alleles in two populations. One locus (Fa6) showed significant deviations from Hardy–Weinberg equilibrium in the Zhaoyang Lake population. By means of Principal Coordinates Analysis, 19 individuals were divided into two groups, which verified they were from two populations. These novel microsatellites will be useful for further studies and genetic analyses of the Eurasian coot.
Additional keyword: genetic diversity.
References
Antos, M. J., Ehmke, G. C., Tzaros, C. L., and Weston, M. A. (2007). Unauthorised human use of an urban coastal wetland sanctuary: current and future patterns. Landscape and Urban Planning 80, 173–183.| Unauthorised human use of an urban coastal wetland sanctuary: current and future patterns.Crossref | GoogleScholarGoogle Scholar |
Dear, E. J., Guay, P. J., Robinson, R. W., and Weston, M. A. (2015). Distance from shore positively influences alert distance in three wetland bird species. Wetlands Ecology and Management 23, 315–318.
| Distance from shore positively influences alert distance in three wetland bird species.Crossref | GoogleScholarGoogle Scholar |
Goldstein, D. B., and Schlöterer, C. (1999). ‘Microsatellites: Evolution and Applications.’ (Oxford University Press.)
Kalinowski, S. T., Taper, M. L., and Marshall, T. C. (2007). Revising how the computer program Cervus, accommodates genotyping error increases success in paternity assignment. Molecular Ecology 16, 1099–1106.
| Revising how the computer program Cervus, accommodates genotyping error increases success in paternity assignment.Crossref | GoogleScholarGoogle Scholar |
Lalitha, S. (2000). Primer Premier 5. Biotech Software & Internet Report 1, 270–272.
| Primer Premier 5.Crossref | GoogleScholarGoogle Scholar |
Loyau, A., and Schmeller, D. S. (2009). Polymorphic microsatellites identified by cross-species amplifications in the European coot Fulica atra. Journal of Ornithology 150, 703–707.
| Polymorphic microsatellites identified by cross-species amplifications in the European coot Fulica atra.Crossref | GoogleScholarGoogle Scholar |
McLeod, E. M., Guay, P. J., Taysom, A. J., Robinson, R. W., and Weston, M. A. (2013). Buses, cars, bicycles and walkers: the influence of the type of human transport on the flight responses of waterbirds. PLoS One 8, e82008.
| Buses, cars, bicycles and walkers: the influence of the type of human transport on the flight responses of waterbirds.Crossref | GoogleScholarGoogle Scholar |
Refseth, U. H., Fangan, B. M., and Jakobsen, K. S. (1997). Hybridization capture of microsatellites directly from genomic DNA. Electrophoresis 18, 1519–1523.
| Hybridization capture of microsatellites directly from genomic DNA.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXmtF2mtrw%3D&md5=4936009d9f797afb774b745d213edb03CAS |
Rousset, F. (2008). Genepop ’007: a complete re-implementation of the Genepop software for Windows and Linux. Molecular Ecology Resources 8, 103–106.
| Genepop ’007: a complete re-implementation of the Genepop software for Windows and Linux.Crossref | GoogleScholarGoogle Scholar |
Schlacher, T. A., Nielsen, T., and Weston, M. A. (2013). Human recreation alters behaviour profiles of non-breeding birds on open-coast sandy shores. Estuarine, Coastal and Shelf Science 118, 31–42.
| Human recreation alters behaviour profiles of non-breeding birds on open-coast sandy shores.Crossref | GoogleScholarGoogle Scholar |
Wang, H., Shi, W., Zhu, L., Luo, X., Chen, Z., and Xie, W. (2010). Isolation and characterization of fourteen polymorphic tetranucleotide microsatellites for Shangcheng stout salamander (Pachyhynobius shangchengensis). Conservation Genetics Resources 2, 121–123.
| Isolation and characterization of fourteen polymorphic tetranucleotide microsatellites for Shangcheng stout salamander (Pachyhynobius shangchengensis).Crossref | GoogleScholarGoogle Scholar |
Zhi, Y. B., Ding, L., Wang, Y., Hu, L., Hua, Y., and Zhao, K. (2014). Isolation and characterization of thirteen novel dinucleotide microsatellite loci from Tetraena mongolica Maxim . Conservation Genetics Resources 6, 297–299.
| Isolation and characterization of thirteen novel dinucleotide microsatellite loci from Tetraena mongolica Maxim .Crossref | GoogleScholarGoogle Scholar |
Zhu, W., Li, H., Zhang, H., Zhang, H., Lv, T., and Xue, S. (2016). Isolation and characterization of twenty-one novel microsatellite loci for the black cicada, Cryptotympana atrata (Hemiptera: Cicadidae). Applied Entomology and Zoology 51, 511–514.
| Isolation and characterization of twenty-one novel microsatellite loci for the black cicada, Cryptotympana atrata (Hemiptera: Cicadidae).Crossref | GoogleScholarGoogle Scholar |
