Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo (Macropus antilopinus)
Jessica J. Wadley A B F , Jeremy J. Austin A B C , Michael G. Gardner D E and Damien A. Fordham BA Australian Centre for Ancient DNA, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia.
B Environment Institute and School of Earth and Environmental Sciences, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia.
C Sciences Department, Museum Victoria, Carlton Gardens, Melbourne, Vic. 3001, Australia.
D School of Biological Sciences, Flinders University, Adelaide, SA 5001, Australia.
E Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5001, Australia.
F Corresponding author. Email: jessica.wadley@adelaide.edu.au
Australian Journal of Zoology 61(5) 399-401 https://doi.org/10.1071/ZO13074
Submitted: 19 September 2013 Accepted: 28 November 2013 Published: 13 December 2013
Abstract
A set of 15 microsatellite loci was optimised for multilocus genotyping of non-invasively collected samples of Macropus antilopinus (antilopine wallaroo). Primers were combined in three PCR multiplexes in order to increase the quality of genotypes from scat samples and to allow for replication. In a screen of 104 scat samples from two populations in north-eastern Australia, three loci were found to be monomorphic while the remaining 12 loci had 2–10 alleles. Genotype frequencies for all 12 microsatellite loci from the two populations did not differ significantly from Hardy–Weinberg equilibrium, and there was no evidence of linkage disequilibrium. These informative markers are specifically designed for non-invasive samples and will be used to assess population structure and conservation genetics of this species in the future.
Additional keywords: faeces, kangaroo, macropod, non-invasive genotyping, population genetics.
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