Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis
Juergen von Zum Hof A , Karsten Schönrogge B , James M. Cook C and Michael G. Gardner A D
+ Author Affiliations
- Author Affiliations
A School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
B Centre for Ecology and Hydrology, CEH Wallingford, Maclean Building, Benson Lane, Wallingford, OX10 8BB, UK.
C School of Biological Sciences, University of Reading, Reading, Berkshire, RG6 6BX, UK.
D Corresponding author. Email: michael.gardner@flinders.edu.au
Australian Journal of Zoology 60(2) 108-110 https://doi.org/10.1071/ZO12042
Submitted: 18 April 2012 Accepted: 25 July 2012 Published: 20 August 2012
Abstract
To date there have been only limited fine-scale investigations into the molecular ecology of the European hoverfly, Microdon mutabilis, due to the paucity of available polymorphic markers. We describe the development of primers amplifying five novel microsatellite loci using next-generation sequencing (454) and three previously undescribed M. mutabilis microsatellite loci using enrichments. In hoverflies from a population in Ireland, the number of alleles per locus ranged from 2 to 16, and the observed heterozygosity ranged between 0.26 and 0.97
Additional keywords: genetic population structure, local adaptation, PCR, social parasite
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