Do experimental methods affect estimates of pollen digestion by birds?
Patricia A. Fleming A B and Tracey L. Moore A
+ Author Affiliations
- Author Affiliations
A School of Veterinary & Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
B Corresponding author. Email: t.fleming@murdoch.edu.au
Australian Journal of Zoology 59(6) 407-415 https://doi.org/10.1071/ZO12016
Submitted: 1 February 2012 Accepted: 5 April 2012 Published: 21 May 2012
Abstract
Pollen protoplasts may supply important nutritional resources for birds; however, they are locked up within the mechanically strong and biochemically complex pollen wall. Previous studies of pollen digestion in birds have yielded highly variable and often contradictory results. We tested whether these differences could reflect the vastly different methodologies that have been used. We used a standard method to investigate digestion of Banksia grandis (Proteaceae) pollen in New Holland honeyeaters (Phylidonyris novaehollandiae). Four types of B. grandis pollen were examined: fresh, frozen for a week, stored in conditions to stimulate pregermination for 24 h, or collected by honeybees. Our data indicate that although pollen treatment may influence digestibility of the pollen grains, these differences do not reach statistical significance because they are dwarfed by a high degree of variability between birds fed the same diet as well as variability in gut transit time (generally more pollen grains were digested over longer transit times). Similar patterns were observed for red wattlebirds (Anthochaera carunculata) fed bee-collected pollen. We believe that feeding behaviour or gut transit time may explain the marked differences between previous studies of pollen digestion by nectarivores, particularly the conflicting results for New Holland honeyeaters.
References
Brice, A. T., Dahl, K. H., and Grau, C. R. (1989). Pollen digestibility by hummingbirds and psittacines. The Condor 91, 681–688.| Pollen digestibility by hummingbirds and psittacines.Crossref | GoogleScholarGoogle Scholar |
Carpenter, F. L., and Castronova, J. L. (1980). Maternal diet selectivity in Calypte anna. American Midland Naturalist 103, 175–179.
| Maternal diet selectivity in Calypte anna.Crossref | GoogleScholarGoogle Scholar |
Churchill, D. M., and Christensen, P. (1970). Observations on pollen harvesting by brush-tongued lorikeets. Australian Journal of Zoology 18, 427–437.
| Observations on pollen harvesting by brush-tongued lorikeets.Crossref | GoogleScholarGoogle Scholar |
Dobson, H. E. M., and Peng, Y. S. (1997). Digestion of pollen components by larvae of the flower-specialist bee Chelostoma florisomne (Hymenoptera: Megachilidae). Journal of Insect Physiology 43, 89–100.
| Digestion of pollen components by larvae of the flower-specialist bee Chelostoma florisomne (Hymenoptera: Megachilidae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXhs1Krs7Y%3D&md5=3d43025268fb68483719ca3fc45eefe4CAS |
Ford, H. A., and Paton, D. C. (1976). The value of insects and nectar to honeyeaters. Emu 76, 83–84.
| The value of insects and nectar to honeyeaters.Crossref | GoogleScholarGoogle Scholar |
Gartrell, B. D. (2000). The nutritional, morphologic, and physiologic bases of nectarivory in Australian birds. Journal of Avian Medicine and Surgery 14, 85–94.
| The nutritional, morphologic, and physiologic bases of nectarivory in Australian birds.Crossref | GoogleScholarGoogle Scholar |
Gartrell, B. D., and Jones, S. M. (2001). Eucalyptus pollen grain emptying by two Australian nectarivorous psittacines. Journal of Avian Biology 32, 224–230.
| Eucalyptus pollen grain emptying by two Australian nectarivorous psittacines.Crossref | GoogleScholarGoogle Scholar |
Grant, B. R. (1996). Pollen digestion by Darwin’s finches and its importance for early breeding. Ecology 77, 489–499.
| Pollen digestion by Darwin’s finches and its importance for early breeding.Crossref | GoogleScholarGoogle Scholar |
Herrera, M. L. G., and Martínez del Río, C. (1998). Pollen digestion by New World bats: effects of processing time and feeding habits. Ecology 79, 2828–2838.
| Pollen digestion by New World bats: effects of processing time and feeding habits.Crossref | GoogleScholarGoogle Scholar |
Human, H., and Nicolson, S. W. (2003). Digestion of maize and sunflower pollen by the spotted maize beetle Astylus atromaculatus (Melyridae): is there a role for osmotic shock? Journal of Insect Physiology 49, 633–643.
| Digestion of maize and sunflower pollen by the spotted maize beetle Astylus atromaculatus (Melyridae): is there a role for osmotic shock?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXkvFyms7k%3D&md5=60a235dddb5c7ff6958ad2b2ba11c16cCAS |
Human, H., and Nicolson, S. W. (2006). Nutritional content of an important South African bee pollen: the fresh, bee-collected and stored pollen of Aloe greatheadii var davyana (Asphodelaceae). Phytochemistry 67, 1486–1492.
| Nutritional content of an important South African bee pollen: the fresh, bee-collected and stored pollen of Aloe greatheadii var davyana (Asphodelaceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xnt1ylsbg%3D&md5=5e2129c5dca0dd2cd0c04b709250b86dCAS |
Johnson, S. A., and Nicolson, S. W. (2001). Pollen digestion in flower-feeding Scarabaeidae: monkey beetles (Hopliini) and protea beetles (Cetoniini). Journal of Insect Physiology 47, 725–733.
| Pollen digestion in flower-feeding Scarabaeidae: monkey beetles (Hopliini) and protea beetles (Cetoniini).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXjsFWgsbk%3D&md5=555fd147dcd50131b6487ddb595c141aCAS |
Kroon, G. H., van Praagh, J. P., and Velthuis, H. H. W. (1974). Osmotic shock as a prerequisite to pollen digestion in the alimentary tract of the worker honeybee. Journal of Apicultural Research 13, 177–181.
Law, B. S. (1992). Physiological factors affecting pollen use by Queensland blossom bats (Syconycteris australis). Functional Ecology 6, 257–264.
| Physiological factors affecting pollen use by Queensland blossom bats (Syconycteris australis).Crossref | GoogleScholarGoogle Scholar |
López-Calleja, M. V., Fernández, M. J., and Bozinovic, F. (2003). The integration of energy and nitrogen balance in the hummingbird Sephanoides sephaniodes. The Journal of Experimental Biology 206, 3349–3359.
| The integration of energy and nitrogen balance in the hummingbird Sephanoides sephaniodes.Crossref | GoogleScholarGoogle Scholar |
Maguire, T. L., and Sedgley, M. (1997). Storage temperature affects viability of Banksia menziesii pollen. HortScience 32, 916–917.
Martínez del Rio, C. (1994). Nutritional ecology of fruit-eating and flower-visiting birds and bats. In ‘The Digestive System in Mammals: Food Form and Function’. (Eds D. Chivers and P. Langer.) pp. 103–127. (Cambridge University Press: Cambridge.)
Nicolson, S. W., and Fleming, P. A. (2003). Nectar as food for birds: the physiological consequences of drinking dilute sugar solutions. Plant Systematics and Evolution 238, 139–153.
Nicolson, S. W., and Thornburg, R. W. (2007). Nectar chemistry. In ‘Nectaries and Nectar’. (Eds S. W. Nicolson, M. Nepi and E. Pacini.) pp. 215–264. (Springer: Dordrecht.)
Pacini, E., and Nicolson, S. W. (2007). Introduction. In ‘Nectaries and Nectar.’ (Eds S. W. Nicolson, M. Nepi and E. Pacini.) pp. 1–18. (Springer: Dordrecht)
Paton, D. C. (1981). The significance of pollen in the diet of the New Holland honeyeater, Phylidonyris novaehollandiae (Aves: Meliphagidae). Australian Journal of Zoology 29, 217–224.
| The significance of pollen in the diet of the New Holland honeyeater, Phylidonyris novaehollandiae (Aves: Meliphagidae).Crossref | GoogleScholarGoogle Scholar |
Recher, H. F. (1977). Ecology of coexisting white-cheeked and New Holland honeyeaters. Emu 77, 136–142.
| Ecology of coexisting white-cheeked and New Holland honeyeaters.Crossref | GoogleScholarGoogle Scholar |
Recher, H. F. (1981). Nectar-feeding and its evolution among Australian vertebrates. In ‘Ecological Biogeography of Australia’. (Ed. A. Keast.) pp. 1639–1648. (W. Junk: The Hague.)
Richardson, K. C., and Wooller, R. D. (1990). Adaptations of the alimentary tracts of some Australian lorikeets to a diet of pollen and nectar. Australian Journal of Zoology 38, 581–586.
| Adaptations of the alimentary tracts of some Australian lorikeets to a diet of pollen and nectar.Crossref | GoogleScholarGoogle Scholar |
Roulston, T. H., and Cane, J. H. (2000). Pollen nutritional content and digestibility for animals. Plant Systematics and Evolution 222, 187–209.
| Pollen nutritional content and digestibility for animals.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXls1Ohu7Y%3D&md5=a5fd78349243e9c1535497f7fb3f5ac7CAS |
Tsahar, E., Arad, Z., Izhaki, I., and Martínez del Río, C. (2006). Do nectar- and fruit-eating birds have lower nitrogen requirements than omnivores? An allometric test. The Auk 123, 1004–1012.
| Do nectar- and fruit-eating birds have lower nitrogen requirements than omnivores? An allometric test.Crossref | GoogleScholarGoogle Scholar |
van Tets, I. G., and Nicolson, S. W. (2000). Pollen and the nitrogen requirements of the lesser double-collared sunbird. The Auk 117, 826–830.
| Pollen and the nitrogen requirements of the lesser double-collared sunbird.Crossref | GoogleScholarGoogle Scholar |
Wooller, R. D., Russell, E. M., and Renfree, M. B. (1983). Technical note: a technique for sampling pollen carried by vertebrates. Australian Wildlife Research 10, 433–434.
| Technical note: a technique for sampling pollen carried by vertebrates.Crossref | GoogleScholarGoogle Scholar |
Wooller, R. D., Richardson, K. C., and Pagendham, C. M. (1988). The digestion of pollen by some Australian birds. Australian Journal of Zoology 36, 357–362.
| The digestion of pollen by some Australian birds.Crossref | GoogleScholarGoogle Scholar |
