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Australian Journal of Zoology Australian Journal of Zoology Society
Evolutionary, molecular and comparative zoology
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RESEARCH ARTICLE
Contents Vol 54(5)

Phylogenetic relationships of the cuscuses and brushtail possums (Marsupialia : Phalangeridae) using the nuclear gene BRCA1

Denise Raterman A C , Robert W. Meredith A , Luis A. Ruedas B and Mark S. Springer A
+ Author Affiliations
- Author Affiliations

A Department of Biology, University of California, Riverside, CA 92521, USA.

B Museum of Vertebrate Biology and Department of Biology, Portland State University, Portland, OR 97207-0751, USA.

C Corresponding author. Email: gansed01@student.ucr.edu

Australian Journal of Zoology 54(5) 353-361 https://doi.org/10.1071/ZO05067
Submitted: 4 November 2005  Accepted: 5 October 2006   Published: 16 November 2006

Abstract

The family Phalangeridae comprises approximately two dozen extinct and extant species that include the brushtail possums (Trichosurus), scaly-tailed possum (Wyulda) and cuscuses (Phalanger, Strigocuscus, Spilocuscus and Ailurops). Morphological studies have suggested that Ailurops ursinus is the sister taxon to all other phalangerids. Another species of interest is Strigocuscus celebensis, whose morphologically based taxonomic affinity has habitually been with trichosurins. Mitochondrial 12S rRNA results, however, found moderate support for an Ailurops and Strigocuscus celebensis clade and placed A. ursinus and S. celebensis as sister to Phalanger and Spilocuscus. This study uses nuclear sequence data from the breast cancer and ovarian cancer susceptibility gene 1 (BRCA1) to test previous mitochondrial DNA results and uses relaxed molecular clock methods to estimate divergence dates. The results support Ailurops as sister taxon to S. celebensis and this clade as sister to Phalangerini. Relaxed molecular-dating methods suggest a date of 23–29 million years for the split between Trichosurini and the remaining phalangerids and 19–24 million years for the split between Ailurops + Strigocuscus celebensis and Phalangerini. Several vicariant/dispersal events are necessary to explain the geographic distribution of the Phalangeridae and our estimated molecular divergence dates are congruent with previously proposed south-east Asian geological events.


Acknowledgments

This work was supported by National Science Foundation grant DEB-9903810 to MSS and National Science Foundation grant DEB-00-75555 to LAR and J. C. Morales.


References

Arnason, U. , Adegoke, J. A. , Bodin, K. , Born, E. W. , Esa, Y. B. , Gullberg, A. , Nilsson, M. , Short, R. V. , Xu, X. , and Janke, A. (2002). Mammalian mitogenomic relationships and the root of the eutherian tree. Proceedings of the National Academy of Sciences of the United States of America 99, 8151–8156.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Crosby K. (2004). Fossil possums in Australasia: discovery, diversity and evolution. In ‘The Biology of Australian Possums and Gliders’. (Eds R. L. Goldingay and S. M. Jackson.) pp. 161–176. (Surrey Beatty: Sydney.)

Crosby, K. , and Norris, C. A. (2003). Periotic morphology in the trichosurin possums Strigocuscus celebensis and Wyulda squamicaudata (Diprotodontia, Phalangeridae) and a revised diagnosis of the tribe Trichosurini. American Museum Novitates 3414, 1–14.
| Crossref | GoogleScholarGoogle Scholar | Drummond A. J., and Rambaut A. (2003). ‘BEAST version 1.3.’ Available at http://evolve.zoo.ox.ac.uk/beast [Verified June 2006].

Drummond, A. J. , Ho, S. Y. W. , Phillips, M. J. , and Rambaut, A. (2006). Relaxed phylogenetics and dating with confidence. PLoS Biology 4, e88.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Flannery T. (1994). ‘Possums of the World. A Monograph of the Phalangeroidea.’ (GEO Productions: Sydney.)

Flannery T., Archer M., and Maynes G. (1987). The phylogenetic relationships of living phalangerids (Phalangeroidea: Marsupialia) with a suggested new taxonomy. In ‘Possums and Opossums: Studies in Evolution’. (Ed. M. Archer.) pp. 477–506. (Surrey Beatty & the Royal Zoological Society of New South Wales: Sydney.)

Flynn, J. J. , and Nedbal, M. A. (1998). Phylogeny of the Carnivora (Mammalia): congruence vs incompatibility among multiple data sets. Molecular Phylogenetics and Evolution 9, 414–426.
| Crossref | GoogleScholarGoogle Scholar | PubMed | George G. G. (1987). Characterization of the living species of cuscus (Marsupialia: Phalangeridae). In ‘Possums and Opossums: Studies in Evolution’. (Ed. M. Archer.) pp. 507–526. (Surrey Beatty & the Royal Zoological Society of New South Wales: Sydney.)

Godthelp, H. , Wroe, S. , and Archer, M. (1999). A new marsupial from the early Eocene Tingamarra Local Fauna of Murgon, southeastern Queensland: a prototypical Australian marsupial? Journal of Mammalian Evolution 6, 289–313.
| Crossref | GoogleScholarGoogle Scholar | Hall R. (1996). Reconstructing Cenozoic SE Asia. In ‘Tectonic Evolution of Southeast Asia’. Special Publication No. 106. (Eds R. Hall and D. Blundell.) pp. 153–184. (Geological Society of London: London.)

Hall R. (1998). The plate tectonics of Cenozoic SE Asia and the distribution of land and sea. In ‘Biogeography and Geological Evolution of SE Asia’. (Eds R. Hall and J. D. Holloway.) pp. 99–131. (Backhuys Publishers: Leiden, the Netherlands.)

Hall, R. (2002). Cenozoic geological and plate tectonic evolution of SE Asia and the SW Pacific: computer-based reconstructions, model and animations. Journal of Asian Earth Sciences 20, 353–431.
| Crossref | GoogleScholarGoogle Scholar | McKenna M. C., and Bell S. K. (1997). ‘Classification of Mammals: Above the Species Level.’ (Columbia University Press: New York.)

Menzies, J. I. , and Pernetta, J. C. (1986). A taxonomic revision of cuscuses allied to Phalanger orientalis Marsupialia Phalangeridae. Journal of Zoology Series B 1, 551–618.
Rambaut A., and Drummond A. J. (2003). ‘Tracer version 1.2.’ Available at http://evolve.zoo.ox.ac.uk [Verified June 2006].

Ronquist, F. , and Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Sorenson M. D. (1999). ‘TreeRot. Version 2.’ (Boston University: Boston, MA.)

Springer, M. S. , Kirsch, J. A. W. , Aplin, K. , and Flannery, T. (1990). DNA hybridization, cladistics, and the phylogeny of phalangerid marsupials. Journal of Molecular Evolution 30, 298–311.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Swofford D. L. (2002). ‘PAUP*. Phylogenetic Analysis Using Parsimony (*And Other Methods). Version 4.’ (Sinauer Associates: Sunderland, MA.)

Swofford D. L., Olsen G. J., Waddell P. J., and Hillis D. M. (1996). Phylogenetic inference. In ‘Molecular Systematics’. 2nd edn. (Eds D. M. Hillis, C. Moritz and B. K. Mable.) pp. 407–514. (Sinauer Associates: Sunderland, MA.)

Tate, G. H. H. (1945). Results of the Archibold Expeditions. No. 52. The marsupial genus Phalanger. American Museum Novitates 1283, 1–30.


Thorne, J. L. , and Kishino, H. (2002). Divergence time and evolutionary rate estimation with multilocus data. Systematic Biology 51, 689–702.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Thorne, J. L. , Kishino, H. , and Painter, I. S. (1998). Estimating the rate of evolution of the rate of molecular evolution. Molecular Biology and Evolution 15, 1647–1657.
| PubMed |

Woodburne, M. O. , MacFadden, B. J. , Case, J. A. , Springer, M. S. , Pledge, N. S. , Power, J. D. , Woodburne, J. M. , and Springer, K. B. (1993). Land mammal biostratigraphy and magnetostratigraphy of the Etadunna Formation (late Oligocene) of South Australia. Journal of Vertebrate Paleontology 13, 483–515.