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RESEARCH ARTICLE
Contents Vol 34(7)

Towards a unique and transmissible vaccine against myxomatosis and rabbit haemorrhagic disease for rabbit populations

Elena Angulo A and Juan Bárcena B C
+ Author Affiliations
- Author Affiliations

A Laboratoire d’Ecologie, Systématique & Evolution, UMR CNRS 8079, Université Paris Sud, 91405 Orsay Cedex, France.

B Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain.

C Corresponding author. Email: barcena@inia.es

Wildlife Research 34(7) 567-577 https://doi.org/10.1071/WR06160
Submitted: 24 November 2006  Accepted: 18 July 2007   Published: 13 December 2007

Abstract

Currently available vaccines against myxomatosis and rabbit hemorrhagic disease virus (RHDV) are not suited to immunise wild rabbit populations, as vaccines need to be delivered individually by conventional veterinary practices. As an alternative approach, research in Spain has focused on the development of a transmissible vaccine. A recombinant virus has been constructed based on a naturally attenuated myxoma virus (MV) field strain, expressing the RHDV capsid protein (VP60). Following inoculation of rabbits, the recombinant virus (MV-VP60) induced specific antibody responses against MV and RHDV, conferring protection against lethal challenges with both viruses. Furthermore, the recombinant MV-VP60 virus showed a limited horizontal transmission capacity, either by direct contact or in a flea-mediated process, promoting immunisation of contact uninoculated animals. Efficacy and safety of the vaccine have been extensively evaluated under laboratory conditions and in a limited field trial. The development of the transmissible vaccine strategy and the steps being taken to obtain the marketing authorisation for the vaccine in the European Union are presented in this review.


Acknowledgements

Elena Angulo acknowledges funding by the ANR-Biodiversité project. The development of the MV-VP60 recombinant vaccine was supported by an agreement between the ‘Fundación para el Estudio y Defensa de la Naturaleza y la Caza’ (FEDENCA) and the ‘Instituto Nacional de Investigación y Tecnologıía Agraria y Alimentaria’ (INIA).


References

Angulo, E. , and Gilna, B. (2008). Crossing the border – international governance of self-dispersive GMOs purposefully released for public health, controlling invasive species and pests, and treating wildlife diseases. Nature Biotechnology ,in press
Delibes M., and Hiraldo F. (1981). The rabbit as prey in the Iberian mediterranean ecosystem. In ‘Proceedings of the World Lagomorph Conference’. (Eds K. Myers and C. D. MacInnes.) pp. 614–622. (University of Guelph: Ontario.)

Delibes-Mateos, M. , RedPath, S , Angulo, E. , Ferreras, P. , and Villafuerte, R. (2007). Rabbits as a keystone species in southern Europe. Biological Conservation 137, 149–156.
| Crossref | GoogleScholarGoogle Scholar | EC (1998). Notice to applicants – veterinary medicinal products. In ‘The Rules Governing Medicinal Products in the European Union. Vol. 6C’. (Office for Official Publications of the European Communities: Luxemburg.)

EC (2001). Directive 2001/18/EC of the European Parliament and of the Council of 12 March 2001 on the deliberate release into the environment of genetically modified organisms and repealing Council Directive 90/220/EEC. Official Journal L106, 1–39.
EMEA (2004). ‘EMEA/CVMP/004/04-final: Guideline on Live Recombinant Vector Vaccines for Veterinary Use.’ (EMEA: London.)

EMEA (2007). ‘SOP/V/4012: Evaluation of Veterinary Medicinal Products Containing or Consisting of Genetically Modified Organisms.’ (EMEA: London.)

Falkner, F. G. , and Moss, B. (1990). Transient dominant selection of recombinant vaccinia viruses. Journal of Virology 64, 3108–3111.
| PubMed | Fenner F., and Fantini B. (1999a). The use of rabbit haemorrhagic disease virus for rabbit control. In ‘Biological Control of Vertebrate Pests – The History of Myxomatosis, an Experiment in Evolution’. pp. 236–272. (CABI Publishing: Oxford.)

Fenner F., and Fantini B. (1999b). ‘Biological Control of Vertebrate Pests – The history of Myxomatosis, an Experiment in Evolution.’ (CABI Publishing: Oxford.)

Fenner F., and Ratcliffe F. N. (1965). ‘Myxomatosis.’ (Cambridge University Press: Cambridge.)

Fenner F., and Ross J. (1994). Myxomatosis. In ‘The European Rabbit. The History and Biology of a Succesful Coloniser’. (Eds H. V. Thompson and C. M. King.) pp. 205–240. (Oxford University Press: Oxford.)

Fenner, F. , and Woodroofe, G. M. (1954). Protection of laboratory rabbits against myxomatosis by vaccination with fibroma virus. Australian Journal of Experimental Biology and Medical Science 32, 653–658.
| PubMed | Ward D. (2005). Reversing rabbit decline – one of the biggest challenges for nature conservation in Spain and Portugal. IUCN Lagomorph Specialist Group. http://www.ualberta.ca/%7Edhik/lsg/field_reports.htm [accessed on June 2007].

Williams K., Parer I., Coman B., Burley J., and Braysher M. (1995). ‘Managing Vertebrate Pests: Rabbits.’ (Bureau of Resource Sciences & CSIRO Division of Wildlife and Ecology & Australian Government Publishing Service: Canberra.)

Wirblich, C. , Thiel, H. J. , and Meyers, G. (1996). Genetic map of the calicivirus Rabbit Hemorrhagic Disease Virus as deduced from in vitro translation studies. Journal of Virology 70, 7974–7983.
| PubMed |