Assessment of errors in nutrient analyses of roots

Abstract

Errors in nutrient analyses of roots may arise from soil adhering to roots, the method of root separation from soil and storage of root samples. Experiments were conducted on fine roots of Eucalyptus nitens from a clay soil to establish a method for estimating true concentrations of nitrogen (N), phosphorus (P) and potassium (K) in root samples (i.e. unbiased by the soil adhering to roots), and to test the adequacy of measurements of ash residues of root samples for estimating the quantity of soil adhering to roots. Results indicated that nutrient concentrations on the basis of ash-free weight of root samples approached true nutrient concentrations of roots when the quality of soil adhering to roots was small, and the nutrient concentration of soil was much lower than the roots. Estimates of true nutrient concentrations of roots calculated from the information on the weight of soil adhering to roots and the nutrient concentration of the soil were satisfactory in the prediction of nutrient content of roots for a range of soil-contamination. The factor which accounted for contamination, and helped estimation of true concentrations from measured concentrations, depended on the magnitude of soil contamination and the relative concentrations of nutrients in roots and soil. Wet separation (washing) of roots from soil compared with dry separation resulted in 24% loss of K. With various methods of storage of washed root samples, the level of soil contamination was 5-20% of the root sample. Submergence of roots in water for 15 days after washing reduced the concentration of N, P and K in roots to 84, 50 and 54% of those roots which were dried immediately following washing. The rate of nutrient loss from roots was greater for K than for N and P when washed samples were stored submerged. On the basis of this study, it is recommended that roots, after separation from soil, should be dried as soon as possible with a minimum exposure of roots to wet conditions. Estimates of soil adhering to roots, and nutrient concentration of the adhering soil, are required to infer correct concentrations of nutrients in root samples.