Measurement of the total water potential of aqueous solutions of polyethylene glycol - A comparison between osmometer, thermocouple psychrometer and equilibrated soil cores

Abstract

This study examined two polyethylene glycol (PEG) polymers (PEG 6000 and PEG 10000) and compared measurements of water potential obtained with a thermocouple osmometer and thermocouple psychrometers at three temperatures (15, 25 and 35°C) and five osmdalities (50, 100, 200, 300 and 400 g/1000 g water). These were then compared with estimates of matric potential of three soils brought to equilibrium with PEG solutions of the same osmolalities. At the same osmolality and temperature the two PEG polymers gave essentially the same water potential. There was a significant effect of temperature on water potential which corresponded closely with changes in specific gravity of the PEG solution. There was a close correlation between the measurements of water potential of the PEG solutions obtained with the osmometer and the psychrometers (R = 0.99). However, the psychrometer gave increasingly lower values than the osmometer as water potential decreased. The differences in the measurements between the two methods are thought to be the result of design and calibration differences. The ease of use of the osmometer is such that it is recommended for routine use. The water potentials of the soil cores brought to equilibrium with the PEG 10 000 solution were linearly related to the water potentials of the PEG solutions estimated from both the osmometer and psychrometers (R2 = 0.84). However, there were clear deviations from a 1:l relationship. It was concluded that the results from the soil cores could not be used to determine which of the two instruments gave the more accurate measurement of water potential of PEG solutions.