Assessment of efficacy of biocides in different soil types for use in sorption studies of low molecular weight organic compounds
Sheridan Martin A , Rai S. Kookana A , Lynne M. Macdonald B and Mark Farrell B C
+ Author Affiliations
- Author Affiliations
A CSIRO Land and Water, Waite Campus, Locked Bag No. 2, Glen Osmond, SA 5064, Australia.
B CSIRO Agriculture and Food, Waite Campus, Locked Bag No. 2, Glen Osmond, SA 5064, Australia.
C Corresponding author. Email: mark.farrell@csiro.au
Soil Research 56(5) 451-455 https://doi.org/10.1071/SR17282
Submitted: 17 October 2017 Accepted: 4 February 2018 Published: 19 April 2018
Abstract
The abiotic protection of low molecular weight organic compounds (LMWOC) in soils may be an important regulator of C cycling. The study of the protection of LMWOC through sorption typically employs soils shaken in solution, which may be compromised by biological activity. We used 14C-labelled glucose as a LMWOC in batch assays of four different soils with contrasting physico-chemical properties (Arenosol, Luvisol, Ferralsol and Andisol). The commonly used biocides NaN3 and HgCl2 were employed alone or in concert across a range of concentrations to assess their efficacy in inhibiting microbial degradation. For short (<1 day) sorption experiments, low NaN3 concentrations (50 µM) were sufficient to inhibit microbial activity, whereas for longer (2 week) equilibrium assays, only high HgCl2 concentrations (10 mM) were effective in all soils tested. All combinations of biocide were most effective in inhibiting microbial activity in the Arenosol and least effective in the Andisol. Thus, in some soils, particular care is required with biocide selection for complete inhibition of biological activity. The findings presented here are of relevance for the design of studies investigating sorption of labile organic compounds in soils.
Additional keywords: abiotic process, LMWOCs, mercuric chloride, radiolabelled compounds, sodium azide, soil organic matter.
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