Priming of soil decomposition leads to losses of carbon in soil treated with cow urine
S. M. Lambie A D , L. A. Schipper B , M. R. Balks B and W. T. Baisden C
+ Author Affiliations
- Author Affiliations
A Landcare Research, Private Bag 3127, Hamilton, New Zealand.
B University of Waikato, Gate 1 Knighton Road, Private Bag 3105, Hamilton 3240, New Zealand.
C GNS Science, National Isotope Centre, PO Box 30–368, Lower Hutt 5040, New Zealand.
D Corresponding author: Email: lambies@landcareresearch.co.nz
Soil Research 51(6) 513-520 https://doi.org/10.1071/SR13148
Submitted: 6 May 2013 Accepted: 20 August 2013 Published: 24 October 2013
Abstract
The extent to which priming of soil carbon (C) decomposition following treatment with cow urine leads to losses of soil C has not been fully investigated. However, this may be an important component of the carbon (C) cycle in intensively grazed pastures. Our objective was to determine soil C losses via priming in soil treated with cow urine and artificial urine. Cow urine, water, 14C-urea artificial urine, and 14C-glucose artificial urine were applied to repacked soil cores and incubated at 25°C for 84 days. We used radio-labelled artificial urine to determine the extent to which urea hydrolysis contributed to elevated carbon dioxide (CO2) emissions in urine-treated soil and as a comparison to the priming effects of cow urine. Water-soluble C, pH, dehydrogenase activity, urease activity, and CO2 evolution were monitored during the incubation. Priming of soil C decomposition (more CO2-C evolved than was added as a C source) in the cow urine treatment was 4.2 ± 0.7 mg C g–1 (5.2 ± 0.9% of soil C concentration, corrected for water control). In the cow urine treatment, ~54% of retained urea was hydrolysed and it contributed 0.4 ± 0.1 mg CO2-C g–1 to total CO2 fluxes. Low urea hydrolysis may have been due to decreased urease activity in the cow urine treatment due to the large amounts of urea present and the increased pH. Dehydrogenase activity was elevated immediately after cow urine application, and indicates that priming was likely due to heightened microbial activity. Negative priming (less CO2-C evolved than was added as a C source) was measured in the artificial urine treatments and this may reflect the differences in composition between the cow and artificial urines. Solubilisation of soil C was also found in the artificial urine treatments, but it did not appear to be correlated with increased pH or periods of greater urea hydrolysis. While cow urine decreased soil C by positively priming soil C decomposition, our artificial urine did not. Therefore, caution is recommended when using artificial urine for C-cycling research. The mechanisms by which both increased soil pH and priming occurs in urine-treated soils require further investigation.
Additional keywords: artificial urine, CO2, dehydrogenase, radio-labelled, urea hydrolysis, urease.
References
Ball R, Kenney DR, Theobald PW, Nes P (1979) Nitrogen balance in urine-affected areas of a New Zealand pasture. Soil Science Society of America Journal 71, 309–314.Blakemore LC, Searle PL, Daly BK (1987) ‘Methods for chemical analysis of soils.’ (NZ Soil Bureau, Department of Scientific and Industrial Research: Lower Hutt, New Zealand)
Clough TJ, Kelliher FM (2005) Dairy farm effluent effects on urine patch nitrous oxide and carbon dioxide emissions. Journal of Environmental Quality 34, 979–986.
| Dairy farm effluent effects on urine patch nitrous oxide and carbon dioxide emissions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXksFykur4%3D&md5=31d3ffe9cefc8d4fe2b5a9559e8ba921CAS | 15888883PubMed |
Clough TJ, Sherlock RR, Kelliher FM (2003) Can liming mitigate N2O fluxes from a urine-amended soil? Australian Journal of Soil Research 41, 439–457.
| Can liming mitigate N2O fluxes from a urine-amended soil?Crossref | GoogleScholarGoogle Scholar |
Dalenberg JW, Jager G (1989) Priming effect of some organic additions to 14C-labelled soil. Soil Biology & Biochemistry 21, 443–448.
| Priming effect of some organic additions to 14C-labelled soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXlslejsrw%3D&md5=3f825a406bbc15228fa6121d2367e171CAS |
Doak BW (1952) Some chemical changes in the nitrogenous constituents of urine when voided on pasture. The Journal of Agricultural Science 42, 162–171.
| Some chemical changes in the nitrogenous constituents of urine when voided on pasture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaG2cXjtlyitw%3D%3D&md5=be83a88f78752fa0e4e73eab8929bf74CAS |
Ghani A, Dexter M, Perrott KW (2003) Hot-water extractable carbon in soils, a sensitive measurement for determining impacts of fertilisation, grazing and cultivation. Soil Biology & Biochemistry 35, 1231–1243.
| Hot-water extractable carbon in soils, a sensitive measurement for determining impacts of fertilisation, grazing and cultivation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXlvVCmtbo%3D&md5=aab940f4458608a58233920f9cd3fbc6CAS |
Harding DE, Ross DJ (1964) Some factors in low-temperature storage influencing the mineralisable-nitrogen of soils. Journal of the Science of Food and Agriculture 15, 829–834.
| Some factors in low-temperature storage influencing the mineralisable-nitrogen of soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF2MXos1SltA%3D%3D&md5=537444fe6608a0fc04d57c2f6dfa2645CAS |
Haynes RJ, Williams PH (1993) Nutrient cycling and soil fertility in the grazed pasture ecosystem. Advances in Agronomy 49, 119–199.
| Nutrient cycling and soil fertility in the grazed pasture ecosystem.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXltlygs7Y%3D&md5=b4b94d025073b591b5861a2b090390eaCAS |
Hewitt AE (1998) ‘New Zealand Soil Classification.’ Landcare Research Science Series No. 1. (Maanaki Whenua Press: Lincoln, New Zealand)
Kool DM, Hoffland E, Abrahamse S, van Groenigen JW (2006) What artificial urine composition is adequate for simulating soil N2O fluxes and mineral N dynamics? Soil Biology & Biochemistry 38, 1757–1763.
| What artificial urine composition is adequate for simulating soil N2O fluxes and mineral N dynamics?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xnt1yltbs%3D&md5=1bb66f5f2d19d54f95bcdd7e61347f3cCAS |
Kuzyakov Y, Bol R (2006) Sources and mechanisms of priming effect induced in two grassland soils amended with slurry and sugar. Soil Biology & Biochemistry 38, 747–758.
| Sources and mechanisms of priming effect induced in two grassland soils amended with slurry and sugar.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XivVaqtrw%3D&md5=d42ba695d58779b7844f49b232490b3bCAS |
Kuzyakov Y, Friedel JK, Stahr K (2000) Review of mechanisms and quantification of priming effects. Soil Biology & Biochemistry 32, 1485–1498.
| Review of mechanisms and quantification of priming effects.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXntFSmu78%3D&md5=cfebf3c6e63ed2e791e0005fabc43356CAS |
Kuzyakov Y, Subbotina I, Chen HB, Bogomolova I, Xu X (2009) Black carbon decomposition and incorporation into soil microbial biomass estimated by 14C labeling. Soil Biology & Biochemistry 41, 210–219.
| Black carbon decomposition and incorporation into soil microbial biomass estimated by 14C labeling.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXotV2isg%3D%3D&md5=ac2d6907effddc6b220e5c3442c04d3bCAS |
Lambie SM (2012) Soil carbon loss under pasture and pine: responses to urine addition. PhD Thesis, University of Waikato, New Zealand.
Lambie SM, Schipper LA, Balks MR, Baisden WT (2012) Carbon leaching from undisturbed soil cores treated with dairy cow urine. Soil Research 50, 320–327.
| Carbon leaching from undisturbed soil cores treated with dairy cow urine.Crossref | GoogleScholarGoogle Scholar |
Lloyd AB, Sheaffe MJ (1973) Urease activity in soils. Plant and Soil 39, 71–80.
| Urease activity in soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3sXltFCnsLw%3D&md5=103adf96b6c090072e1e67c19d0690c7CAS |
Lockyer DR (1984) A system for the measurement in the field of losses of ammonia through volatisation. Journal of the Science of Food and Agriculture 35, 837–848.
Lovell RD, Jarvis SC (1996) Effects of urine on soil microbial biomass, methanogenesis, nitrification and denitrification. Plant and Soil 186, 265–273.
| Effects of urine on soil microbial biomass, methanogenesis, nitrification and denitrification.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXntVWjsg%3D%3D&md5=4d7aece01a5fa629602689ee77bb36bfCAS |
MacLeod CJ, Moller H (2006) Intensification and diversification of New Zealand agriculture since 1960, An evaluation of current indicators of land use change. Agriculture, Ecosystems & Environment 115, 201–218.
| Intensification and diversification of New Zealand agriculture since 1960, An evaluation of current indicators of land use change.Crossref | GoogleScholarGoogle Scholar |
Marsh WH, Fingerhut B, Miller H (1965) Automated and manual direct methods for the determination of blood urea. Clinical Chemistry 11, 624–627.
Marsh KL, Sims GK, Mulvaney RL (2005) Availability of urea to autotrophic ammonia-oxidizing bacteria as related to the fate of 14C- and 15N-labeled urea added to soil. Biology and Fertility of Soils 42, 137–145.
Moir JL, Cameron KC, Di HJ, Fertsak U (2011) The spatial coverage of dairy cattle urine patches in an intensively grazed pasture system. The Journal of Agricultural Science 149, 473–485.
| The spatial coverage of dairy cattle urine patches in an intensively grazed pasture system.Crossref | GoogleScholarGoogle Scholar |
Murphy DV, Sparling GP, Fillery IRP (1998) Stratification of microbial biomass C and N and gross N mineralisation with soil depth in two contrasting Western Australian agricultural soils. Australian Journal of Soil Research 36, 45–55.
| Stratification of microbial biomass C and N and gross N mineralisation with soil depth in two contrasting Western Australian agricultural soils.Crossref | GoogleScholarGoogle Scholar |
Orwin KH, Bertram JE, Clough TJ, Condron LM, Sherlock RR, O’Callaghan M, Ray J, Baird DB (2010) Impact of bovine urine deposition on soil microbial activity, biomass, and community structure. Applied Soil Ecology 44, 89–100.
| Impact of bovine urine deposition on soil microbial activity, biomass, and community structure.Crossref | GoogleScholarGoogle Scholar |
Parliamentary Commissioner for the Environment (2004) ‘Growing for Good. Intensive farming, sustainability and New Zealand’s environment.’ (Parliamentary Commissioner for the Environment: Wellington, New Zealand)
Pleasants AB, Shorten PR, Wake GC (2007) The distribution of urine deposited on a pasture from grazing animals. The Journal of Agricultural Science 145, 81–86.
| The distribution of urine deposited on a pasture from grazing animals.Crossref | GoogleScholarGoogle Scholar |
Rachhpal-Singh , Nye PH (1984) The effect of soil pH and high urea concentrations on urease activity in soil. Journal of Soil Science 35, 519–527.
Rooney D, Kennedy N, Deering L, Gleeson D, Clipson N (2006) Effect of sheep urine deposition on the bacterial community structure in an acidic upland grassland soil. Applied and Environmental Microbiology 72, 7231–7237.
| Effect of sheep urine deposition on the bacterial community structure in an acidic upland grassland soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1GksrzJ&md5=879a33d0baacaf0b06b6a3e0c0b78e2cCAS | 17088382PubMed |
Ross DJ (1971) Some factors influencing the estimation of dehydrogenase activities of some soils under pasture. Soil Biology & Biochemistry 3, 97–110.
| Some factors influencing the estimation of dehydrogenase activities of some soils under pasture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3MXkvFOksrY%3D&md5=591d99e0c6d472c52fbf7e8892fd63f8CAS |
Saggar S, Parshotam A, Hedley C, Salt G (1999) 14C-labelled glucose turnover in New Zealand soils. Soil Biology & Biochemistry 31, 2025–2037.
| 14C-labelled glucose turnover in New Zealand soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXntVOiur8%3D&md5=1632be351466397e93d89985bf62949dCAS |
Schipper LA, Baisden WT, Parfitt RL, Ross C, Claydon JJ, Arnold GC (2007) Large losses of soil C and N from soil profiles under pasture in New Zealand during the past 20 years. Global Change Biology 13, 1138–1144.
| Large losses of soil C and N from soil profiles under pasture in New Zealand during the past 20 years.Crossref | GoogleScholarGoogle Scholar |
Schipper LA, Parfitt RL, Ross C, Baisden WT, Claydon JJ, Fraser S (2010) Gains and losses in C and N stocks of New Zealand pasture soils depend on land use. Agriculture, Ecosystems & Environment 139, 611–617.
| Gains and losses in C and N stocks of New Zealand pasture soils depend on land use.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjs1eitA%3D%3D&md5=03a407384656ddf33f7dc0c7db398d3cCAS |
Sherlock RR, Goh KM (1984) Dynamics of ammonia volatization from simulated urine patches and aqueous urea applied to pasture. I. Field experiments. Fertilizer Research 5, 181–195.
Skujiņš J (1976) Extracellular enzymes in soil. CRC Critical Reviews in Microbiology 4, 383–421.
| Extracellular enzymes in soil.Crossref | GoogleScholarGoogle Scholar |
Soil Survey Staff (2011) ‘Keys to Soil Taxonomy.’ 11th edn (USDA-Natural Resources Conservation Service: Washington, DC)
Sparling GP, Parfitt RL, Hewitt AE, Schipper LA (2003) Three approaches to define desired soil organic matter contents. Journal of Environmental Quality 32, 760–766.
Sparling GP, Wheeler D, Vessely ET, Schipper LA (2006) What is soil organic matter worth? Journal of Environmental Quality 35, 548–557.
| What is soil organic matter worth?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XisFKrurw%3D&md5=c82ab7574285188022c4895c48b980e0CAS | 16510699PubMed |
Stillwell MA, Woodmansee RG (1981) Chemical transformations of urea-nitrogen and movement of nitrogen in a shortgrass prairie soil. Soil Science Society of America Journal 45, 893–898.
| Chemical transformations of urea-nitrogen and movement of nitrogen in a shortgrass prairie soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL38XltFanug%3D%3D&md5=fc38404642c392eb92a827e227345d15CAS |
Tabatabai MA (1994) Soil enzymes. In ‘Methods of soil analysis, Part 2. Microbiological and biochemical properties’. (Eds RW Weaver, S Angle, P Bottomley, D Bezdicek, S Smith, A Tabatabai) pp. 775–833. (Soil Science Society of America: Madison, WI)
Tabatabai MA, Bremner JM (1972) Assay of urease activity in soils. Soil Biology & Biochemistry 4, 479–487.
| Assay of urease activity in soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3sXivVertg%3D%3D&md5=91eda93e1bcba735326eb1ea41ffffaeCAS |
Uchida Y, Clough TJ, Kelliher FM, Hunt JE, Sherlock RR (2011) Effects of bovine urine, plants and temperature on N2O and CO2 emissions from a sub-tropical soil. Plant and Soil 345, 171–186.
| Effects of bovine urine, plants and temperature on N2O and CO2 emissions from a sub-tropical soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXptFymt7k%3D&md5=74c0c1fc752582a702507bb920ce5b77CAS |
Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass. Soil Biology & Biochemistry 19, 703–707.
| An extraction method for measuring soil microbial biomass.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXjs1KqsA%3D%3D&md5=744b63f3d4f9c062abfa4faa04452309CAS |
Zantua MI, Bremner JM (1976) Production and persistence of urease activity in soils. Soil Biology & Biochemistry 8, 369–374.
| Production and persistence of urease activity in soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE28XlslKjt7Y%3D&md5=ba503e7252baf7ba9ca20879c3feea1eCAS |
