Influence of temperature and soil type on inhibition of urea hydrolysis by N-(n-butyl) thiophosphoric triamide in wheat and pasture soils in south-eastern Australia
H. C. Suter A D , P. Pengthamkeerati A B , C Walker C and D. Chen A
+ Author Affiliations
- Author Affiliations
A Melbourne School of Land and Environment, The University of Melbourne, Vic. 3010, Australia.
B Environmental Technology Research Unit (EnviTech), Department of Environmental Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
C Incitec Pivot Limited, PO Box 54, North Geelong, Vic. 3215, Australia.
D Corresponding author. Email: helencs@unimelb.edu.au
Soil Research 49(4) 315-319 https://doi.org/10.1071/SR10243
Submitted: 8 November 2010 Accepted: 23 February 2011 Published: 19 May 2011
Abstract
Incubation experiments were conducted to assess the effectiveness of N-(n-butyl) thiophosphoric triamide (NBPT) for inhibiting hydrolysis of urea in three wheat-growing soils and one pasture soil in south-eastern Australia, under a range of temperatures (5, 15, 25°C). The effectiveness of NBPT decreased with increasing temperature and with increasing urease activity. In the acidic pasture soil with high urease activity (186 μg N/g soil.h) and high organic carbon content (11%), NBPT (0.1% w/w urea) had little impact on urea hydrolysis rates over all temperatures, with <1% urea remaining at Day 14. In the alkaline, wheat-cropping soils with lower urease activity (54–90 μg N/g soil.h) and lower organic carbon content (<1.5%), NBPT was able to effectively reduce urea hydrolysis over 14–15 days at 5°C and 15°C (>55% urea remaining). At 25°C in the wheat soils, NBPT slowed the rate of urea hydrolysis, but by Days 14 and 15, <2% of the urea remained. NBPT applied at a rate of 0.1% urea would be an effective tool for slowing urea hydrolysis in the wheat-cropping soils under cool-climate conditions. The delay in urea hydrolysis in the pasture soil still provides the opportunity for increased flexibility in farm management, such as irrigation scheduling.
Additional keywords: incubation studies, NBPT, urease activity.
References
Antisari LV, Marzadori C, Gioacchini P, Ricci S, Gessa C (1996) Effects of the urease inhibitor N-(n-butyl) phosphorothioic triamide in low concentrations on ammonia volatilization and evolution of mineral nitrogen. Biology and Fertility of Soils 22, 196–201.| Effects of the urease inhibitor N-(n-butyl) phosphorothioic triamide in low concentrations on ammonia volatilization and evolution of mineral nitrogen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28Xkt1Klt7c%3D&md5=3bec92e2ccba5d9e90dcd9464c8491cbCAS |
Bremner JM, McCarty GW, Yeomans JC, Chai HS (1986) Effects of phosphoroamides on nitrification, denitrification, and mineralization of organic nitrogen in soil. Communications in Soil Science and Plant Analysis 17, 369–384.
| Effects of phosphoroamides on nitrification, denitrification, and mineralization of organic nitrogen in soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28Xkt1emt7k%3D&md5=97c46247e5b823da8b5aafb0a229e90bCAS |
Cai G, Chen D, White RE, Fan XH, Pacholski A, Zhu ZL, Ding H (2002) Gaseous nitrogen losses from urea applied to maize on a calcareous fluvo-aquic soil in the North China Plain. Australian Journal of Soil Research 40, 737–748.
| Gaseous nitrogen losses from urea applied to maize on a calcareous fluvo-aquic soil in the North China Plain.Crossref | GoogleScholarGoogle Scholar |
Carmona G, Christianson CB, Byrnes BH (1990) Temperature and low concentration effects of the urease inhibitor N-(n-butyl) thiophosphoric triamide (nBTPT) on ammonia volatilization from urea. Soil Biology & Biochemistry 22, 933–937.
| Temperature and low concentration effects of the urease inhibitor N-(n-butyl) thiophosphoric triamide (nBTPT) on ammonia volatilization from urea.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXotVCnsg%3D%3D&md5=66e98f0e3cc7158601557650d9a63059CAS |
Chai HS, Bremner JM (1987) Evaluation of some phosphoroamides as soil urease inhibitors. Biology and Fertility of Soils 3, 189–194.
| Evaluation of some phosphoroamides as soil urease inhibitors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2sXlvFKqt78%3D&md5=1792f66b79af54928314e10a18ca8fb7CAS |
Chen D, Suter HC, Islam A, Edis R, Freney JR, Walker CN (2008) Prospects of improving efficiency of fertiliser nitrogen in Australian agriculture: a review of enhanced efficiency fertilisers. Australian Journal of Soil Research 46, 289–301.
| Prospects of improving efficiency of fertiliser nitrogen in Australian agriculture: a review of enhanced efficiency fertilisers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXns1OktLw%3D&md5=eb7d08453a8e9922f2dff8c61faf711eCAS |
Christianson CB, Howard RG (1994) Use of soil thin-layer chromatography to assess the mobility of the phosphoric triamide urease inhibitors and urea in soil. Soil Biology & Biochemistry 26, 1161–1164.
| Use of soil thin-layer chromatography to assess the mobility of the phosphoric triamide urease inhibitors and urea in soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXlvVGltw%3D%3D&md5=8daa3b5a92a0078f707edbf32dcf4b15CAS |
Dalal RC (1985) Distribution, salinity, kinetic and thermodynamic characteristics of urease activity in a vertisol profile. Australian Journal of Soil Research 23, 49–60.
| Distribution, salinity, kinetic and thermodynamic characteristics of urease activity in a vertisol profile.Crossref | GoogleScholarGoogle Scholar |
Eckard RJ, Chen D, White RE, Chapman DF (2003) Gaseous nitrogen loss from temperate perennial grass and clover dairy pastures in south-eastern Australia. Australian Journal of Agricultural Research 54, 561–570.
| Gaseous nitrogen loss from temperate perennial grass and clover dairy pastures in south-eastern Australia.Crossref | GoogleScholarGoogle Scholar |
FAO (2006) FAOSTAT Database Collections/ResourceSTAT/Fertilizers Archive/Australia-Urea-1961/. Available at: http://faostat.fao.org/site/422/DesktopDefault.aspx?PageID=422#ancor
Hendrickson LL, Douglass EA (1993) Metabolism of the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) in soils. Soil Biology & Biochemistry 25, 1613–1618.
| Metabolism of the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) in soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXjtVWrtQ%3D%3D&md5=e42c696e61eb929a5c46392362bdf6faCAS |
Hornik K (2009) The R Project for Statistical Computing. Available at: http://CRAN.R-project.org
IFA (2009) International Fertilizer Industry Association/statistics/IFAData/ Australia-Urea-Consumption-2008. Available at: www.fertilizer.org/ifa/ifadata/search
Karamanos RE, Harapiak JT, Flore NA, Stonehouse TB (2004) Use of N-(n-butyl) thiophosphoric triamide (NBPT) to increase safety of seed-placed urea. Canadian Journal of Plant Science 84, 105–116.
Kiss S, Simihaian M (2002) ‘Improving efficiency of urea fertilizers by inhibition of soil urease activity.’ (Kluwer Academic Publishers: The Netherlands)
Kumar V, Wagenet RJ (1984) Urease activity and kinetics of urea transformation in soils. Soil Science 137, 263–269.
| Urease activity and kinetics of urea transformation in soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXktVKjsLY%3D&md5=ce8feae9ac37c3a87f6dcbe9c8c92b9dCAS |
Medina R, Radel RJ (1988) Mechanisms of urease inhibition. In ‘Ammonia volatilization from urea fertilizers’. (Eds BR Bock, DE Kissel) pp. 137–174. (National Fertilizer Development Program: Muscle Shoals)
Moyo CC, Kissel DE, Cabrera ML (1989) Temperature effects on soil urease activity. Soil Biology & Biochemistry 21, 935–938.
| Temperature effects on soil urease activity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXitVemsw%3D%3D&md5=5ccb262303911a2f68ebd9bc0ed85a0cCAS |
Rawluk CDL, Grant CA, Racz GJ (2001) Ammonia volatilization from soils fertilized with urea and varying rates of urease inhibitor NBPT. Canadian Journal of Soil Science 81, 239–246.
Roy AH, Hammond LL (2004) Challenges and opportunities for the fertilizer industry. In ‘Agriculture and the nitrogen cycle: assessing the impacts of fertilizer use on food production and the environment’. (Eds AR Mosier, JK Syers, JR Freney) pp. 233–243. (Island Press: Washington, DC)
Singh J, Saggar S, Bolan NS (2004) Mitigating gaseous losses of nitrogen from pasture soil with urease and nitrification inhibitors. In ‘SuperSoil 2004: 3rd Australian New Zealand Soils Conference’. 5–9 December 2004, University of Sydney, Australia. (Ed. B Singh) (The Regional Institute: Gosford, NSW)
Skalar Analytical BV (2005) ‘The SANplus Segmented Flow Analyser: Soil and Plant analysis.’ (Skalar Analytical B.V.: The Netherlands)
Trasar-Cepeda C, Gil-Sotres F, Leirós MC (2007) Thermodynamic parameters of enzymes in grassland soils from Galicia, NW Spain. Soil Biology & Biochemistry 39, 311–319.
| Thermodynamic parameters of enzymes in grassland soils from Galicia, NW Spain.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1agtrzL&md5=d6331422ec3fd6b06a34d009df6ca433CAS |
Turner DA, Edis RB, Chen D, Freney JR, Denmead OT, Christie R (2010) Determination and mitigation of ammonia loss from urea applied to winter wheat with N-(n-butyl) thiophosphorictriamide. Agriculture, Ecosystems & Environment 137, 261–266.
| Determination and mitigation of ammonia loss from urea applied to winter wheat with N-(n-butyl) thiophosphorictriamide.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXltFOms7k%3D&md5=4ffa5c934fe3209dd2c27c8dd016b8c9CAS |
Wang Z, Cleemput O, Demeyer P, Baert L (1991) Effect of urease inhibitors on urea hydrolysis and ammonia volatilization. Biology and Fertility of Soils 11, 43–47.
| Effect of urease inhibitors on urea hydrolysis and ammonia volatilization.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXlvVajurY%3D&md5=c0f60c2def6110b9dc5bf2a4e5d11c81CAS |
Wang Z, Cleemput OV, Baert L (1996) Movement of urea and its hydrolysis products as influenced by moisture content and urease inhibitors. Biology and Fertility of Soils 22, 101–108.
| Movement of urea and its hydrolysis products as influenced by moisture content and urease inhibitors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XkslKhurc%3D&md5=9d3aaa1e9ca4786f2f248aaf685e9792CAS |
Watson CJ (2005) Urease inhibitors. In ‘IFA International Workshop on Enhanced-Efficiency Fertilizers’. Frankfurt, Germany, 28–30 June 2005. (International Fertilizer Industry Association) Available at: www.fertilizer.org/ifa/
Watson CJ, Akhonzada NA, Hamilton JTG, Matthews DI (2008) Rate and mode of application of the urease inhibitor N-(n-butyl) thiophosphoric triamide on ammonia volatilization from surface-applied urea. Soil Use and Management 24, 246–253.
| Rate and mode of application of the urease inhibitor N-(n-butyl) thiophosphoric triamide on ammonia volatilization from surface-applied urea.Crossref | GoogleScholarGoogle Scholar |
Watson CJ, Miller H (1996) Short-term effects of urea amended with the urease inhibitor N-(n-butyl) thiophosphoric triamide on perennial ryegrass. Plant and Soil 184, 33–45.
| Short-term effects of urea amended with the urease inhibitor N-(n-butyl) thiophosphoric triamide on perennial ryegrass.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXlsVKgsw%3D%3D&md5=937b61e416ad9cc4ad6fb1f7651ced87CAS |
Xu JG, Heeraman DA, Wang Y (1993) Fertilizer and temperature effects on urea hydrolysis in undisturbed soil. Biology and Fertility of Soils 16, 63–65.
| Fertilizer and temperature effects on urea hydrolysis in undisturbed soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXmsVyqtr4%3D&md5=061fe0ed8bebe36a2829db12a7982596CAS |
