Effects of increasing salinity and 15N-labelled urea levels on growth, N uptake, and water use efficiency of young tomato plants

Abstract

A greenhouse experiment was conducted to investigate the response of tomato plants (Lycopersicon lycopersicum L.) to salinity and to determine the interactive effects of salinity and nitrogen fertilisation on yield, nitrogen uptake, water use efficiency (WUE), and root-zone salinity during early plant growth. Furthermore, the effects of salinity and N fertilisation were evaluated by measurement of carbon isotope discrimination (Δ). Tomato plants were grown in pots filled with 8 kg (dry weight equivalent) of Krumbach sandy loam. Salinity treatments were imposed by irrigation water containing Na, Ca, and Mg salts and having electrical conductivity of 0, 3, 6, 9, and 12 dS/m at 25°C. ¹⁵N-labelled urea (10 atom % excess) was also applied at 0, 80, 160, and 240 mg N/kg soil. Increasing salinity reduced plant growth; fresh and dry weights of shoots and roots decreased significantly, except for the non-fertilised plants. The maximum growth reduction in shoots occurred due to salinity–N fertilisation relationships at 12 dS/m (59.4% reduction compared with 0 dS/m in 160 mg N/kg). Root growth was less affected than shoots. Vegetative growth and N content increased with increasing nitrogen treatment. However, salinity generally reduced N uptake by plants. Δ was negatively correlated with WUE at all salinity levels in young tomato plants. Similar correlations were also obtained between WUE and Δ at various N treatments; the result suggests that Δ is a useful tool for assessing stress conditions. Smaller Δ values were obtained when salinity or N level increased. Increasing N fertiliser increased WUE in plants, whereas increasing salinity increased WUE at 3 dS/m and decreased WUE to some extent at other salinity levels. Electrical conductivity of the root-zone increased due to increasing salinity and time, whereas pH decreased. It was concluded that the early stage of development was a salt sensitive period for tomato plants.