Root effects on soil carbon and nitrogen cycling in a Pinus radiata D. Don plantation on a coastal sand

Abstract

Although the contribution of roots to soil carbon (C) fluxes and biochemical processes is recognised, it is difficult to quantify. One approach to assess their importance is the use of trenched plots, in which C inputs to the soil and respiration by living roots has ceased. We give here an account of C and nitrogen (N) pools and mineralisation in samples taken 27 months after trenching in a 26-year-old Pinus radiata D. Don plantation on a coastal sand (an Aquic Udipsamment); above-ground litter inputs continued throughout the 27-month period.Moisture contents were higher in FH material and mineral soil from the trenched than from the control plots. Trenching had no effect on total organic C and N concentrations, but led to decreases in extractable C, microbial C and N, and CO₂-C production values at some depths in the soil profile. Mineral-N concentrations and gross nitrification rates were, in contrast, initially higher in the trenched-plot samples, but were similar in both treatments after incubation of the samples at 25°C for 57 days. Mineral-N concentrations were also higher in the trenched than control mineral soil after in situ incubation.

On an area basis (to 20 cm depth of mineral soil), inputs from roots were estimated to account for about 40% of the extractable C pool, 28% of microbial C, 26% of microbial N, and 23% of heterotrophic CO₂-C production (0–7 days at 25°C) in the control soil.

Overall, our results suggest a tight connection between N cycling rates and the labile C pools derived from below-ground inputs, with nitrification in particular increasing as C availability declined as a result of trenching.