Seed micromorphology of central European Orobanche and Phelipanche (Orobanchaceae) in relation to preferred hosts and systematic implications
Renata Piwowarczyk
+ Author Affiliations
- Author Affiliations
Department of Botany, Institute of Biology, Jan Kochanowski University, 15 Świętokrzyska Street, PL-25-406 Kielce, Poland. Email: renata.piwowarczyk@ujk.edu.pl
Australian Systematic Botany 28(3) 124-136 https://doi.org/10.1071/SB15007
Submitted: 12 March 2015 Accepted: 16 June 2015 Published: 13 November 2015
Abstract
Holoparasitic genera within family Orobanchaceae are characterised by greatly reduced vegetative organs; therefore, seed micromorphology has proved to be a useful complementary taxonomic criterion. Seeds of 160 samples from 54 localities of 26 taxa of the Orobanche and Phelipanche genera occurring in central Europe, specifically from Poland, the Czech Republic, Austria and Slovakia, supplemented by samples from Spain, France and Ukraine, were investigated using scanning electron microscopy. Thirteen quantitative or qualitative morphological characters of seeds were analysed. The following three types of periclinal wall sculpture of seeds were identified: veined and fibrillar in Phelipanche; with oval or elliptic perforations (pitted) in almost all species of Orobanche; with outer periclinal wall smooth, granular or rugged (very rarely visibly pitted), impeding vision of the inner one, occurring only in O. gracilis Sm. and O. coerulescens Stephan in Willd. The influence of different hosts on the features of seeds of eight species is also presented, as well as relationships between seed morphology and taxonomic classification, including problematic taxa. The best diagnostic features include type of ornamentation of the periclinal wall, perforation diameter (in pitted sculpture), fibrillar diameter (in fibrillar sculpture) and width of anticlinal walls. Size and shape of the seeds and cells and the presence of median troughs are variable; however, these features can be helpful when using larger samples. The usefulness of micromorphological studies on seeds of Orobanche and Phelipanche is demonstrated.
Additional keywords: Orobanche, Phelipanche, seeds, host, SEM, systematic significance.
References
Abu Sbaih HA, Jury SL (1994) Seed micromorphology and taxonomy in Orobanche (Orobanchaceae). Flora Mediterranea 4, 41–48.Barthlott W (1981) Epidermal and seed surface characters of plants: systematic applicability and some evolutionary aspects. Nordic Journal of Botany 1, 345–355.
| Epidermal and seed surface characters of plants: systematic applicability and some evolutionary aspects.Crossref | GoogleScholarGoogle Scholar |
Beck von Mannagetta G (1930) Orobanchaceae L. In ‘Das Pflanzenreich (IV.261)’. (Ed. A Engler) (Leipzig)
Carlón L, Gómez Casares G, Laínz M, Moreno Moral G, Sánchez Pedraja Ó, Schneeweiss GM (2008) Más, a propósito de algunas Phelipanche Pomel, Boulardia F.W.Schultz y Orobanche L. (Orobanchaceae) del oeste del Paleártico. Documentos Jardín Botánico Atlántico (Gijón) 6, 1–128.
Deif HAR, Al-Menoufi OA, Ahmed MF (2000) A taxonomic study of the populations of three common species of the genus Orobanche L. (Orobanchaceae) in Egypt. 2. Macro- and micromorphology of the seeds and their taxonomic implication. In ‘Plant Systematics for the 21st Century’, (Eds B Nordenstam, G El-Ghazaly, M Kassas) pp. 251–266. (Portland Press; London)
Domina G (2009) Orobanchaceae. p. 331. In: Greuter W, Raab-Straube E (2009) Euro+Med Notulae, 4. Willdenowia 39, 327–333.
Domina G, Colombo P (2005) Seed micromorphology and its variation in Sicilian Orobanche (Magnoliopsida). Flora Mediterranea 15, 99–107.
Gower JC (1971) A general coefficient of similarity and some of its properties. Biometrics 27, 857–874.
| A general coefficient of similarity and some of its properties.Crossref | GoogleScholarGoogle Scholar |
Halamski AT (2011) Orobanche hederae Vaucher ex Duby (Orobanchaceae): phenotypic plasticity and seed micromorphology. Bulletin Mensuel de la Societe Linneenne de Lyon 80, 195–203.
Halamski AT, Piwowarczyk R (2008) Graines d’Orobanches comme critère taxonomique: information sur les travaux en cours. Bulletin Mensuel de la Societe Linneenne de Lyon 77, 37–40.
Hayek A (1914) Orobanchaceae. In ‘Illustrierte Flora von Mitteleuropa, vol. 6’. (Ed. G Hegi) (München)
Holub J, Zázvorka J (1999) Phelipanche bohemica. In ‘Cervena kniha ohrozenych a vzacnych druhu rostlin a zivocichu CR a SR. Vol. 5’. (Eds J Cerovsky, V Ferakova, J Holub, S Maglocky, F Prochazka) p. 279. (Priroda: Bratislava, Slovakia)
Joel DM, Bar H, Mayer AM, Plakhine D, Ziadne H, Westwood JH, Welbaum GE (2012) Seed ultrastructure and water absorption pathway of the root-parasitic plant Phelipanche aegyptiaca (Orobanchaceae). Annals of Botany 109, 181–195.
| Seed ultrastructure and water absorption pathway of the root-parasitic plant Phelipanche aegyptiaca (Orobanchaceae).Crossref | GoogleScholarGoogle Scholar | 22025523PubMed |
Joel DM, Gressel J, Musselman LJ (Eds) (2013) ‘Parasitic Orobanchaceae: parasitic mechanisms and control strategies.’ (Springer: Heidelberg, Germany)
Kovach WL (1999) ‘MVSP: a multivariate statistical package for Windows, ver. 3.1.’ (Kovach Computing Services: Pentraeth, Wales, UK)
Kreutz CAJ (1995) ‘Orobanche. The European broomrape species. Central and northern Europe.’ (Natuurhistorisch Genootschap: Maastricht, Netherlands)
Krupp A, Rücker E, Heller A, Spring O (2014) Seed structure characteristics of Orobanche cumana populations. Helia 38, 1–14.
| Seed structure characteristics of Orobanche cumana populations.Crossref | GoogleScholarGoogle Scholar |
Łomnicki A (2005) ‘Wprowadzenie do statystyki dla przyrodników.’ (Wydawnictwo Naukowe PWN: Warszawa, Poland)
Manen JF, Habashi C, Jeanmonod D, Park JM, Schneeweiss GM (2004) Phylogeny and intraspecific variability of holoparasitic Orobanche (Orobanchaceae) inferred from plastid rbcL sequences. Molecular Phylogenetics and Evolution 33, 482–500.
| Phylogeny and intraspecific variability of holoparasitic Orobanche (Orobanchaceae) inferred from plastid rbcL sequences.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXntVKqu7Y%3D&md5=b289dc898ed59c2a533ece4d231720a2CAS | 15336681PubMed |
McNeal JR, Bennett JR, Wolfe AD, Mathews S (2013) Phylogeny and origins of holoparasitism in Orobanchaceae. American Journal of Botany 100, 971–983.
| Phylogeny and origins of holoparasitism in Orobanchaceae.Crossref | GoogleScholarGoogle Scholar | 23608647PubMed |
Musselman LJ, Mann WF (1976) A survey of surface characteristics of seeds of Scrophulariaceae and Orobanchaceae using scanning electron microscopy. Phytomorphology 26, 370–378.
Olsen S, Deyrup Olsen I (1980) The seed of Boschniakia hookeri (Orobanchaceae). Botanisk Tidskrift 75, 159–172.
Park JM, Manen JF, Schneeweiss GM (2007a) Horizontal gene transfer of a plastid gene in the non-photosynthetic flowering plants Orobanche and Phelipanche (Orobanchaceae). Molecular Phylogenetics and Evolution 43, 974–985.
| Horizontal gene transfer of a plastid gene in the non-photosynthetic flowering plants Orobanche and Phelipanche (Orobanchaceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXlvVOru7c%3D&md5=e3943c1e257f0667bfe7869c48973469CAS | 17116411PubMed |
Park J, Schneeweiss G, Weiss-Schneeweiss H (2007b) Diversity and evolution of Ty1-copia and Ty3-gypsy retroelements in the nonphotosynthetic flowering plants Orobanche and Phelipanche (Orobanchaceae). Gene 387, 75–86.
| Diversity and evolution of Ty1-copia and Ty3-gypsy retroelements in the nonphotosynthetic flowering plants Orobanche and Phelipanche (Orobanchaceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtlCmtL%2FF&md5=5135a016ab0b316c22f3f9778b117df9CAS | 17008031PubMed |
Piednoël M, Aberer AJ, Schneeweiss GM, Macas J, Novak P, Gundlach H, Temsch EM, Renner SS (2012) Next-generation sequencing reveals the impact of repetitive DNA across phylogenetically closely related genomes of Orobanchaceae. Molecular Biology and Evolution 29, 3601–3611.
| Next-generation sequencing reveals the impact of repetitive DNA across phylogenetically closely related genomes of Orobanchaceae.Crossref | GoogleScholarGoogle Scholar | 22723303PubMed |
Piwowarczyk R (2011) Orobanche mayeri (Suess. & Ronniger) Bertsch & F. Bertsch: the new species to Poland. Acta Societatis Botanicorum Poloniae 80, 179–183.
| Orobanche mayeri (Suess. & Ronniger) Bertsch & F. Bertsch: the new species to Poland.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R (2012a) Orobanche alba subsp. alba and subsp. major (Orobanchaceae) in Poland: current distribution, taxonomy, plant communities, hosts, and seed micromorphology. Biodiversity: Research and Conservation 26, 23–38.
Piwowarczyk R (2012b) Orobanche bohemica Čelak. (Orobanchaceae) at the eastern limit of its geographical range: new data on its distribution in Poland. Biodiversity: Research and Conservation 26, 53–59.
Piwowarczyk R (2013) Seed productivity in relation to other schoot features for endangered parasitic plant Orobanche picridis F.W. Schultz (Orobanchaceae). Polish Journal of Ecology 61, 55–64.
Piwowarczyk R (2014) Orobanche caryophyllacea Sm. (Orobanchaceae) in Poland: current distribution, taxonomy, plant communities and hosts. Acta Agrobotanica 67, 97–118.
| Orobanche caryophyllacea Sm. (Orobanchaceae) in Poland: current distribution, taxonomy, plant communities and hosts.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R (2015) Orobanche zajaciorum (Orobanchaceae): a new species from the Caucasus. Phytotaxa 201, 214–220.
| Orobanche zajaciorum (Orobanchaceae): a new species from the Caucasus.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R, Jankowska-Błaszczuk M (2014) Intra-specific diversity of seed productivity and morphological features in parasitic species Orobanche bartlingii Griseb. (Orobanchaceae). Polish Journal of Ecology 62, 723–738.
Piwowarczyk R, Krajewski Ł (2015) Orobanche elatior and O. kochii (Orobanchaceae) in Poland: distribution, taxonomy, plant communities and seed micromorphology. Acta Societatis Botanicorum Poloniae 84, 103–123.
| Orobanche elatior and O. kochii (Orobanchaceae) in Poland: distribution, taxonomy, plant communities and seed micromorphology.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R, Przemyski A (2009) New locality of Orobanche coerulescens Stephan ex Willd. (Orobanchaceae) at the NW limit of its geographical range. Acta Societatis Botanicorum Poloniae 78, 291–295.
| New locality of Orobanche coerulescens Stephan ex Willd. (Orobanchaceae) at the NW limit of its geographical range.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R, Przemyski A (2010) The distribution and habitat preferences of the declining species Orobanche arenaria (Orobanchaceae) at the northern limit of its geographical range. Acta Societatis Botanicorum Poloniae 79, 43–50.
| The distribution and habitat preferences of the declining species Orobanche arenaria (Orobanchaceae) at the northern limit of its geographical range.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R, Chmielewski P, Gierczyk B, Piwowarski B, Stachyra P (2010) Orobanche pallidiflora Wimm. & Grab. in Poland: distribution, habitat and host preferences. Acta Societatis Botanicorum Poloniae 79, 197–205.
| Orobanche pallidiflora Wimm. & Grab. in Poland: distribution, habitat and host preferences.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R, Chmielewski P, Cwener A (2011) The distribution and habitat requirements of the genus Orobanche L. (Orobanchaceae) in SE Poland. Acta Societatis Botanicorum Poloniae 80, 37–48.
| The distribution and habitat requirements of the genus Orobanche L. (Orobanchaceae) in SE Poland.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R, Halamski AT, Durska E (2014) Seed and pollen morphology in the Orobanche alsatica complex (Orobanchaceae) from central Europe and its taxonomic significance. Australian Systematic Botany 27, 145–157.
| Seed and pollen morphology in the Orobanche alsatica complex (Orobanchaceae) from central Europe and its taxonomic significance.Crossref | GoogleScholarGoogle Scholar |
Piwowarczyk R, Madeja J, Nobis M (2015) Pollen morphology of the central European broomrapes (Orobanchaceae: Orobanche, Phelipanche and Orobanchella) and its taxonomical implications. Plant Systematics and Evolution 301, 795–808.
| Pollen morphology of the central European broomrapes (Orobanchaceae: Orobanche, Phelipanche and Orobanchella) and its taxonomical implications.Crossref | GoogleScholarGoogle Scholar |
Plaza L, Fernandez I, Juan R, Pastor J, Pujadas A (2004) Micromorphological studies on seeds of Orobanche species from the Iberian Peninsula and the Balearic Islands, and their systematic significance. Annals of Botany 94, 167–178.
| Micromorphological studies on seeds of Orobanche species from the Iberian Peninsula and the Balearic Islands, and their systematic significance.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2czhsFyltw%3D%3D&md5=7e67056b9fef1588391a095f35261c4dCAS | 15159219PubMed |
Pujadas-Salvà AJ (2007) Novedades taxonómicas y nomenclaturalers en el género Orobanche L. (Orobanchaceae). Acta Botanica Malacitana 32, 1–3.
Pusch J (2006) Die Böhmische Sommerwurz (Orobanche bohemica Čelak.): ein Beitrag zur Abgrenzung, Verbreitung und Gefährdung dieses zentraleuropäischen Endemiten. Veröffentlichungen des Naturkundemuseum Erfurt 25, 127–148.
Pusch J, Günther KF (2009) Orobanchaceae (Sommerwurzgewächse). In ‘Illustrierte Flora von Mitteleuropa’. (Ed. G Hegi) pp. 1–99. (Weissdorn-Verlag: Jena, Germany)
Salisbury EJ (1942) ‘The reproductive capacity of plants.’ (Bell & Sons: London)
Schneeweiss GM (2007) Correlated evolution of life history and host range in the nonphotosynthetic parasitic flowering plants Orobanche and Phelipanche (Orobanchaceae). Journal of Evolutionary Biology 20, 471–478.
| Correlated evolution of life history and host range in the nonphotosynthetic parasitic flowering plants Orobanche and Phelipanche (Orobanchaceae).Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2s7gvVKlsA%3D%3D&md5=d438c6ecaa82eed1d6e96066a7c78bc2CAS | 17305812PubMed |
Schneeweiss GM, Colwell A, Park JM, Jang CG, Stuessy TF (2004a) Phylogeny of holoparasitic Orobanche (Orobanchaceae) inferred from nuclear ITS sequence. Molecular Phylogenetics and Evolution 30, 465–478.
| Phylogeny of holoparasitic Orobanche (Orobanchaceae) inferred from nuclear ITS sequence.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXjtVGitA%3D%3D&md5=a2a12f9dff1cccaf97916e3014f2aa52CAS | 14715236PubMed |
Schneeweiss GM, Palomeque T, Colwell A, Weiss-Schneeweiss H (2004b) Chromosome numbers and karyotype evolution of holoparasitic Orobanche (Orobanchaceae) and related genera. American Journal of Botany 91, 439–448.
| Chromosome numbers and karyotype evolution of holoparasitic Orobanche (Orobanchaceae) and related genera.Crossref | GoogleScholarGoogle Scholar | 21653400PubMed |
Shahi Shavvon R, Saeidi Mehrvarz S (2010) Pollen and seed morphology of the genus Cistanche (Orobanchaceae) in Iran. Biologia 65, 615–620.
| Pollen and seed morphology of the genus Cistanche (Orobanchaceae) in Iran.Crossref | GoogleScholarGoogle Scholar |
Sokal R, Michener C (1958) A statistical method for evaluating systematic relationships. The University of Kansas Science Bulletin 38, 1409–1438.
Stearn WT (1992). ‘Botanical Latin.’ (David & Charles: London)
Teryokhin ES (1997). ‘Weed Broomrapes: Systematics, Ontogenesis, Biology, Evolution.’ (Aufstieg-Verlag: Landshut, Germany)
Teryokhin ES, Shibakina GV, Serafimovich NB, Kravtsova T (1993) ‘Determinator of Broomrapes of the USSR flora.’ (Nauka: St Petersburg, Russia)
Tiagi B (1951) Studies in the family Orobanchaceae. III. A contribution to the embryology of Orobanche cernua Loeffl. and O. aegyptiaca Pers. Phytomorphology 1, 158–169.
Velasco L, Goffman FG, Pujadas A (2000) Fatty acids and tocochromanols in seeds of Orobanche. Phytochemistry 54, 295–300.
| Fatty acids and tocochromanols in seeds of Orobanche.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXkt1Sjurg%3D&md5=4bc51fe597b2fde27b0029832ebaf64aCAS | 10870184PubMed |
Weiss-Schneeweiss H, Greilhuber J, Schneeweiss GM (2006) Genome size evolution in holoparasitic Orobanche (Orobanchaceae) and related genera. American Journal of Botany 93, 148–156.
| Genome size evolution in holoparasitic Orobanche (Orobanchaceae) and related genera.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xpt1Cqug%3D%3D&md5=9e977fce3039620a352db4e6d22cd1d0CAS |
Zare G, Dönmez AA (2013) Two new records of the genus Orobanche (Orobanchaceae) from Turkey. Turkish Journal of Botany 37, 1–7.
Zare G, Dönmez AA, Dönmez EO (2014) Pollen morphology and evolution in the genus Orobanche L. s.l. and its allied genera (Orobancheae/Orobanchaceae) in Turkey. Plant Systematics and Evolution 300, 783–802.
| Pollen morphology and evolution in the genus Orobanche L. s.l. and its allied genera (Orobancheae/Orobanchaceae) in Turkey.Crossref | GoogleScholarGoogle Scholar |
Zázvorka J (1997) Orobanchaceae Vent.: záarazovite. In ‘Flóra Slovenska. Vol. 5’. (Ed. K Goliašová) pp. 460–529. (Veda: Bratislava, Slovakia)
Zázvorka J (2000) Orobanchaceae Vent.: zarazovite. In ‘Kvétena České Republiky. Vol. 6’. (Ed. B Slavík) pp. 477–513. (Academia: Prague, Czech Republic)
Zázvorka J (2010) Orobanche kochii and O. elatior (Orobanchaceae) in central Europe. Acta Musei Moraviae. Scientiae Biologicae 95, 77–119.
